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Agroindustrial byproduct-based media in the production of microbial oil rich in oleic acid and carotenoids

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Abstract

This study focuses on the potential of Rhodotorula mucilaginosa CCT 7688 in simultaneous production of lipids and carotenoids in agroindustrial byproduct-based media and specially aims at establishing a process condition that guarantees high concentrations of both bioproducts, i.e., a carotenoid-rich microbial oil with potential economic value and health benefits attributed to carotenoids and fatty acids. Four different combinations of cultivation modes (batch and fed-batch) and alternative substrates (crude glycerol, sugarcane molasses and corn steep liquor) were tested. The M2-B assay, which comprises the use of an agroindustrial byproduct-based medium without any supplementation (70 g L−1 sugarcane molasses and 3.4 g L−1 corn steep liquor) and batch mode, was selected as the most promising one to produce both compounds. Total carotenoid production and total lipid content were 1794.2 µg L−1 and 43.2% (w/w), respectively, after 144 h of cultivation. The fatty acid profile showed predominance of oleic acid (69.9%) and palmitic acid (23.2%). Thus, R. mucilaginosa CCT 7688 may be used in simultaneous production of lipids and carotenoids successfully; its fatty acid profile is similar to that found in olive oil. Both compounds are economically interesting and have great possibility of future commercial applications.

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Acknowledgements

The authors are grateful for FAPERGS (Foundation Research Support in the State of Rio Grande do Sul), CNPq (National Council of Science and Technological Development). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.

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  1. Sensory Analysis and Quality Control Laboratory, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, 96203-900, Brazil

    Tábita Veiga Dias Rodrigues, Erika Carvalho Teixeira, Luana Pinheiro Macedo, Gabriel Maio dos Santos, Carlos André Veiga Burkert & Janaína Fernandes de Medeiros Burkert

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  1. Tábita Veiga Dias Rodrigues
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Rodrigues, T.V.D., Teixeira, E.C., Macedo, L.P. et al. Agroindustrial byproduct-based media in the production of microbial oil rich in oleic acid and carotenoids. Bioprocess Biosyst Eng 45, 721–732 (2022). https://doi.org/10.1007/s00449-022-02692-1

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