Abstract
Phytosterols are natural plant–based bioactive compound shaving various roles in human health that are widely used in the food, nutrition, pharmaceutical, and cosmetics industries. Phytosterol extraction and isolation techniques are difficult and time consuming. The growing demand for phytosterols encourages the development of easy-to-use technologies for extracting and isolating them from various plant sources. Free phytosterols extracted from plants are extensively used in fortified meals and nutritional supplements. Bioactivities of phytosterols have sparked interest in obtaining them from vegetable oils or industrial wastes. The growing demand for these bioactive compounds as a food supplement may spur additional advancements in extraction, isolation, and analytical processes that are more efficient, quick, and environmentally friendly. This review summarizes both conventional and non-conventional extraction techniques of phytosterols and their use in the food industry. Supercritical fluid extraction is the emerging technique to extract phytosterols due to its efficiency; however, further experiments are needed to create optimal working conditions and instruments.
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Mustafa AM, Abouelenein D, Acquaticci L, Alessandroni L, Angeloni S, Borsetta G, Caprioli G, Nzekoue FK, Sagratini G, Vittori S (2022) Polyphenols, saponins and phytosterols in lentils and their health benefits: an overview. Pharmaceuticals 15:1225
Nattagh-Eshtivani E, Barghchi H, Pahlavani N, Barati M, Amiri Y, Fadel A, Khosravi M, Talebi S, Arzhang P, Ziaei R (2022) Biological and pharmacological effects and nutritional impact of phytosterols: a comprehensive review. Phyther Res 36:299–322
Mohammadi M, Jafari SM, Hamishehkar H, Ghanbarzadeh B (2020) Phytosterols as the core or stabilizing agent in different nanocarriers. Trends Food Sci Technol 101:73–88. https://doi.org/10.1016/j.tifs.2020.05.004
Shahbaz MU, Arshad M, Mukhtar K, Nabi BG, Goksen G, Starowicz M, Nawaz A, Ahmad I, Walayat N, Manzoor MF, Aadil RM (2022) Natural plant extracts: an update about novel spraying as an alternative of chemical pesticides to extend the postharvest shelf life of fruits and vegetables. Molecules 27(16):5152. https://doi.org/10.3390/molecules27165152
de Paulo Farias D, de Araújo FF, Neri-Numa IA, Pastore GM (2019) Prebiotics: trends in food, health and technological applications. Trends Food Sci Technol 93:23–35
A Sahar, U ur Rahman, A Ishaq, MS Munir, RM Aadil (2019) Health-promoting perspectives of fruit-based functional energy beverages, in: Sport. Energy Drink., Elsevier, pp 399–439. https://doi.org/10.1016/b978-0-12-815851-7.00012-7
Riaz A, Aadil RM, Amoussa AMO, Bashari M, Abid M, Hashim MM (2021) Application of chitosan-based apple peel polyphenols edible coating on the preservation of strawberry (Fragaria ananassa cv Hongyan) fruit. J Food Process Preserv 45(1), e15018. https://doi.org/10.1111/jfpp.15018
Žlabur JS, Voća S, Brnč M, Rimac-Brnč S (2018) New trends in food technology for green recovery of bioactive compounds from plant materials. In: Role of materials science in food bioengineering. Academic Press, pp 1–36
Shahzad N, Khan W, Md S, Ali A, Saluja SS, Sharma S, Al-Allaf FA, Abduljaleel Z, Ibrahim IAA, Abdel-Wahab AF, Afify MA, Al-Ghamdi SS (2017) Phytosterols as a natural anticancer agent: current status and future perspective. Biomed Pharmacother 88:786–794. https://doi.org/10.1016/j.biopha.2017.01.068
Salehi B, Quispe C, Sharifi-Rad J, Cruz-Martins N, Nigam M, Mishra AP, Konovalov DA, Orobinskaya V, Abu-Reidah IM, Zam W, Sharopov F, Venneri T, Capasso R, Kukula-Koch W, Wawruszak A, Koch W (2020) Phytosterols: from preclinical evidence to potential clinical applications. Front Pharmacol 11:599959. https://doi.org/10.3389/fphar.2020.599959
Andualem M (2023) Nutritional and anti-nutritional characteristics of okra (Abelmoschus esculents (l.) Moench) accessions grown in Pawe district, northwestern Ethiopia. Int J Agri Biosci 12:18–21
Bangulzai N, Ahmed SF, Kashif M, Fatima M, Ahmed M, Mushtaq N (2022) Antifungal activity of essential oils extracted from different plants against Penicillium digitatum causing green mold of citrus. Int J Agri Biosci 11:75–83
Zhang X, Lin K, Li Y (2020) Highlights to phytosterols accumulation and equilibrium in plants: biosynthetic pathway and feedback regulation. Plant Physiol Biochem 155:637–649. https://doi.org/10.1016/j.plaphy.2020.08.021
Nyström L, Schär A, Lampi A (2012) Steryl glycosides and acylated steryl glycosides in plant foods reflect unique sterol patterns. Eur J Lipid Sci Technol 114:656–669
Ismail HTH (2022) Toxic impact of exposure to calcium hypochlorite and granular activated carbon on African catfish (Clarias gariepinus): a study of the alterations in hemato-biochemical profile and oxidative indices. Int J Vet Sci 11(2):129–140. https://doi.org/10.47278/journal.ijvs/2021.082
El-Motaily NM, Farag HS, Abdou OM, Saber M, Ahmed KA (2022) The relationship between antioxidant trace elements (Zn-Cu and Se) and oxidative stress in dogs affected with dermatophytosis. Int J Vet Sci 11(4):467–473
Leong W, Man Y, Lai OM, Long K, Misran M, Tan C (2009) Optimization of processing parameter’s for the preparation of phytosterol microemulsions by the solvent displacement method. J Agric Food Chem 57:8426–8433. https://doi.org/10.1021/jf901853y
Almeida CAS, Baggio SR, Mariutti LRB, Bragagnolo N (2020) One-step rapid extraction of phytosterols from vegetable oils. Food Res Int 130:108891. https://doi.org/10.1016/j.foodres.2019.108891
F Tf, A-E Ghany, S Morsy, H Hassan, A Samy (2023) Evaluation of olive leaves and pomace extracts in growing rabbit diets on productive performance, nutrient digestibility, carcass characteristics, antioxidant status and economic efficiency. Int J Vet Sci . https://doi.org/10.47278/journal.ijvs/2022.155
Mohamed MA, Hassan HMA (2023) Phytogenic substances as safe growth promoters in poultry nutrition. Int J Vet Sci 12:89–100
N Dedhia, N Shah (2023) Polyphenols, phytosterols, aromatics, and essential oils, in: Eng. Plant-Based Food Syst. Elsevier 255–275
Norhazlindah MF, Jahurul MHA, Norliza M, Shihabul A, Islam S, Nyam KL, Zaidul ISM (2023) Techniques for extraction, characterization, and application of oil from sacha inchi (Plukenetia volubilis L) seed: a review. J Food Meas Charact 17:904–915
JafarianAsl P, Niazmand R, Yahyavi F (2020) Extraction of phytosterols and tocopherols from rapeseed oil waste by supercritical CO2 plus co-solvent: a comparison with conventional solvent extraction. Heliyon. 6:e03592. https://doi.org/10.1016/j.heliyon.2020.e03592
García-González A, Velasco J, Velasco L, Ruiz-Méndez MV (2021) Characterization of press and solvent extraction oils from new sunflower seeds with modified phytosterol compositions. J Sci Food Agric 101:101–109. https://doi.org/10.1002/jsfa.10619
Poulose N, Sajayan A, Ravindran A, Chandran A, Priyadharshini GB, Selvin J, Kiran GS (2021) Anti-diabetic potential of a stigmasterol from the seaweed Gelidium spinosum and its application in the formulation of nanoemulsion conjugate for the development of functional biscuits. Front Nutr 8:694362. https://doi.org/10.3389/fnut.2021.694362
Jaski JM, Barbosa Abrantes KK, Zanqui AB, Stevanato N, da Silva C, Barão CE, Bonfim-Rocha L, Cardozo-Filho L (2022) Simultaneous extraction of sunflower oil and active compounds from olive leaves using pressurized propane. Curr Res Food Sci. 5:531–544. https://doi.org/10.1016/j.crfs.2022.03.002
Zeng J, Xiao T, Ni X, Wei T, Liu X, Deng ZY, Li J (2022) The comparative analysis of different oil extraction methods based on the quality of flaxseed oil. J Food Compos Anal 107:104373. https://doi.org/10.1016/j.jfca.2021.104373
D. Bozdoğan Konuşkan (2020) Minor bioactive lipids in cold pressed oils. Cold Press Oils 7–14. https://doi.org/10.1016/b978-0-12-818188-1.00002-5
Naebi M, Torbati M, Azadmard-Damirchi S, Siabi S, Savage GP (2022) Changes in physicochemical properties of cold press extracted oil from Balangu (Lallemantia peltata) seeds during storage. J Food Compos Anal 107:104358. https://doi.org/10.1016/j.jfca.2021.104358
Mingyai S, Srikaeo K, Kettawan A, Singanusong R, Nakagawa K, Kimura F, Ito J (2018) Effects of extraction methods on phytochemicals of rice bran oils produced from colored rice. J Oleo Sci 67:135–142. https://doi.org/10.5650/jos.ess17122
Zhang Y, Zhou Y, Song Z, Jin J, Tang J, Wang X, Huang J, Jin Q (2021) A chemometrics approach comparing characteristics and free radical scavenging capacity of flax (Linum usitatissimum L) oils obtained from seeds and cakes with different extraction methods. J Sci Food Agric 101:5359–5367. https://doi.org/10.1002/jsfa.11184
Cheng C, Yu K, Yu X, Geng F, Huang F, Wang L, Huang Q, Quan S, Deng Q (2022) Optimized endogenous lipid concomitants in flaxseed oil by different oil extraction technologies: their positive roles in emulsions. LWT 155:113000. https://doi.org/10.1016/j.lwt.2021.113000
Aguirre MR, Velasco J, Ruiz-Méndez MV (2014) Characterization of sunflower oils obtained separately by pressing and subsequent solvent extraction from a new line of seeds rich in phytosterols and conventional seeds. OCL 21(6):D605
Sangpradab J, Kamonpatana P, Suwannaporn P, Huang TC (2021) Ohmic heating-aided mechanical extraction of gamma-oryzanol and phytosterols in rice bran oil. Food Bioprocess Technol 14:1542–1554. https://doi.org/10.1007/s11947-021-02655-6
Özcan MM, Ghafoor K, Al Juhaimi F, Ahmed IAM, Babiker EE (2019) Effect of cold-press and soxhlet extraction on fatty acids, tocopherols and sterol contents of the Moringa seed oils, South African. J. Bot. 124:333–337. https://doi.org/10.1016/j.sajb.2019.05.010
Fithriani D (2019) Nurhayati, Optimization of the condition of phytosterol extraction conditions from microalgae nannochloropsis using ethanol of different purity levels. J Bio-Science 27:143–148. https://doi.org/10.3329/jbs.v27i0.44679
Marques FG, de Oliveira Neto JR, da Cunha LC, de Paula JR, Bara MTF (2015) Identification of terpenes and phytosterols in Dipteryx alata (baru) oil seeds obtained through pressing. Rev Bras Farmacogn 25:522–525. https://doi.org/10.1016/j.bjp.2015.07.019
Mildner-Szkudlarz S, Różańska M, Siger A, Kowalczewski PŁ, Rudzińska M (2019) Changes in chemical composition and oxidative stability of cold-pressed oils obtained from by-product roasted berry seeds. Lwt 111:541–547. https://doi.org/10.1016/j.lwt.2019.05.080
Narváez-Cuenca CE, Inampues-Charfuelan ML, Hurtado-Benavides AM, Parada-Alfonso F, Vincken JP (2020) The phenolic compounds, tocopherols, and phytosterols in the edible oil of guava (Psidium guava) seeds obtained by supercritical CO2 extraction. J Food Compos Anal 89:103467. https://doi.org/10.1016/j.jfca.2020.103467
Alvarez-Henao MV, Cardona L, Hincapié S, Londoño-Londoño J, Jimenez-Cartagena C (2022) Supercritical fluid extraction of phytosterols from sugarcane bagasse: evaluation of extraction parameters. J Supercrit Fluids 179:105427. https://doi.org/10.1016/j.supflu.2021.105427
Asl PJ, Niazmand R, Jahani M (2020) Theoretical and experimental assessment of supercritical CO2 in the extraction of phytosterols from rapeseed oil deodorizer distillates. J Food Eng 269:109748. https://doi.org/10.1016/j.jfoodeng.2019.109748
Nyam KL, Tan CP, Lai OM, Long K, Che Man YB (2010) Optimization of supercritical fluid extraction of phytosterol from roselle seeds with a central composite design model, Food Bioprod. Process 88:239–246. https://doi.org/10.1016/j.fbp.2009.11.002
Ekinci M, Gürü M (2019) Extraction of phytosterols from melon (Cucumis melo) seeds by supercritical CO2 as a clean technology. Green Process Synth 8:677–682. https://doi.org/10.1515/gps-2019-0038
Hrabovski N, Sinadinović-Fišer S, Nikolovski B, Sovilj M, Borota O (2012) Phytosterols in pumpkin seed oil extracted by organic solvents and supercritical CO 2. Eur J Lipid Sci Technol 114:1204–1211. https://doi.org/10.1002/ejlt.201200009
Santos KA, Klein EJ, Fiorese ML, Palú F, da Silva C, da Silva EA (2020) Extraction of Morus alba leaves using supercritical CO2 and ultrasound-assisted solvent: evaluation of β-sitosterol content. J Supercrit Fluids 159:104752. https://doi.org/10.1016/j.supflu.2020.104752
Silva D, Anastassia DA, Natália F, Silva C (2019) Sunflower oil from enzymatic aqueous extraction process: maximization of free oil yield and oil characterization. J Food Process Eng 42(6):e13169. https://doi.org/10.1111/jfpe.13169
Feng S, Wang L, Shao P, Lu B, Chen Y, Sun P (2022) Simultaneous analysis of free phytosterols and phytosterol glycosides in rice bran by SPE/GC-MS. Food Chem 387:132742. https://doi.org/10.1016/j.foodchem.2022.132742
Feng S, Wang L, Belwal T, Li L, Luo Z (2020) Phytosterols extraction from hickory (Carya cathayensis Sarg.) husk with a green direct citric acid hydrolysis extraction method. Food Chem 315:126217. https://doi.org/10.1016/j.foodchem.2020.126217
Noormazlinah N, Hashim N, Nour AH, Abdul Munaim MS, Almajano MP, Bahirah N (2019) Extraction of phytosterol concentration in different legume pods by using microwave-assisted hydrodistillation. Indones J Chem 19:796. https://doi.org/10.22146/ijc.40865
Hu B, Zhou K, Liu Y, Liu A, Zhang Q, Han G, Liu S (2018) Industrial crops & products optimization of microwave-assisted extraction of oil from tiger nut (Cyperus esculentus L.) and its quality evaluation. Ind Crop Prod 115:290–297. https://doi.org/10.1016/j.indcrop.2018.02.034
Stevanato N, da Silva C (2019) Radish seed oil: Ultrasound-assisted extraction using ethanol as solvent and assessment of its potential for ester production. Ind Crops Prod 132:283–291. https://doi.org/10.1016/j.indcrop.2019.02.032
Tolve R, Condelli N, Can A, Tchuenbou-Magaia FL (2018) Development and characterization of phytosterol-enriched oil microcapsules for foodstuff application. Food Bioprocess Technol 11:152–163. https://doi.org/10.1007/s11947-017-1990-4
Poudel A, Gachumi G, Wasan KM, Bashi ZD, El Aneed A, Badea I (2019) Development and characterization of liposomal formulations containing phytosterols extracted from canola oil deodorizer distillate along with tocopherols as food additives. Pharmaceutics 11(4):185. https://doi.org/10.3390/pharmaceutics11040185
da Silva Santos V, Braz BB, Silva AÁ, Cardoso LP, Ribeiro APB, Santana MHA (2019) Nanostructured lipid carriers loaded with free phytosterols for food applications. Food Chem 298:125053. https://doi.org/10.1016/j.foodchem.2019.125053
Bagherpour S, Alizadeh A, Ghanbarzadeh S, Mohammadi M, Hamishehkar H (2017) Preparation and characterization of Betasitosterol-loaded nanostructured lipid carriers for butter enrichment. Food Biosci 20:51–55. https://doi.org/10.1016/j.fbio.2017.07.010
Goh A, Ningtyas D, Bhandari B, Prakash S (2021) Investigating phytosterol as a potential functional component in milk through textural, flavour and oral perception study. LWT 141:110873. https://doi.org/10.1016/j.lwt.2021.110873
Ningtyas DW, Bhandari B, Bansal N, Prakash S (2018) Texture and lubrication properties of functional cream cheese: effect of β-glucan and phytosterol. J Texture Stud 49:11–22. https://doi.org/10.1111/jtxs.12282
Amaral JBS, Grisi CVB, Vieira EA, Ferreira PS, Rodrigues CG, Diniz NCM, Vieira PPF, dos Santos NA, Gonçalves MC, Braga ALM, de Cordeiro AMTM (2022) Light cream cheese spread of goat milk enriched with phytosterols: physicochemical, rheological, and microbiological characterization. LWT. 157:113103. https://doi.org/10.1016/j.lwt.2022.113103
Nzekoue AF, Alesi A, Vittori S, Sagratini G, Caprioli G (2020) Development of a functional whey cheese (ricotta) enriched in phytosterols: evaluation of the suitability of whey cheese matrix and processing for phytosterols supplementation. LWT- Food Sci Technol 139:L110479. https://doi.org/10.1016/j.lwt.2020.110479
Ahangari H, Yousefi M, Abedi R, Mirzanajafi‐Zanjani M, Aman Mohammadi M, Ehsani A, Moghaddas Kia E (2021) Probiotic Ayran development by incorporation of phytosterols and microencapsulated Lactobacillus casei L26 in sodium caseinate–gellan mixture. Int J Dairy Technol 75(1):150–158. https://doi.org/10.1111/1471-0307.12812
Seyhan E, Yaman H, Ozer B (2015) Production of a whey-based functional beverage supplemented with soy isoflavones and phytosterols. Int J Dairy Technol 69(1):114–121. https://doi.org/10.1111/1471-0307.12229
Nagaraj V, Nath B, Arora S, Naik L (2018) Fortification of milk with phytosterol and its effect on sensory and physicochemical properties. Irish J Agric Food Ressearch 57:63–70. https://doi.org/10.1515/ijafr-2018-0007
Guo Q, Li T, Qu Y, Wang X, Liu L, Liu H, Wang Q (2021) Action of phytosterols on thermally induced trans fatty acids in peanut oil. Food Chem 344:128637. https://doi.org/10.1016/j.foodchem.2020.128637
Ben Moussa O, Mzoughi M, Chouaibi M, Boulares M, Hassouna M (2019) The combined effect of phytosterols and lactulose supplementation on yoghurt quality. J Food Nutri Res 7:261–269. https://doi.org/10.12691/jfnr-7-4-2
Comunian T, Chaves I, Thomazini M, Moraes I, Furtado R, Castro I, Favaro-Trindade C (2017) Development of functional yogurt containing free and encapsulated echium oil, phytosterol and sinapic acid. Food Chem 237:948–956. https://doi.org/10.1016/j.foodchem.2017.06.071
Tolve R, Condelli N, Caruso M, Favati F, Barletta D, Galgano F (2018) Fortification of dark chocolate with microencapsulated phytosterols: chemical and sensory evaluation. Food Funct 9(2):1265–1273. https://doi.org/10.1039/C7FO01822C
Chen X, Chen Y, Jinmei W, Guo J, Yin S-W, Yang X (2016) Phytosterols structured algae oil nanoemulsions and powders: Improving antioxidant and flavor properties. Food Funct 7(9):3694–3702. https://doi.org/10.1039/C6FO00449K
Hussain M, Li X, Liu L, Wang L, Qayum A, Purevsuren B, Hussain A (2020) Characterization and anti-hyper-lipidemic effect of micro encapsulated phytosterol enriched cheddar cheese. LWT 134:110114. https://doi.org/10.1016/j.lwt.2020.110114
Ferguson J, Wolska A, Remaley A, Stojanovski E, MacDonald-Wicks L, Garg M (2019) Bread enriched with phytosterols with or without curcumin modulates lipoprotein profiles in hypercholesterolaemic individuals. A randomised controlled trial. Food Funct 10:2515–2527. https://doi.org/10.1039/c8fo02512f
Zhao YR, Chen YP, Cheng YF, Qu HM, Li J, Wen C, Zhou YM (2019) Effects of dietary phytosterols on growth performance, antioxidant status, and meat quality in Partridge Shank chickens. Poult Sci 98:3715–3721. https://doi.org/10.3382/ps/pez059
Naji TA, Amadou I, Abbas S, Zhao R-Y, Shi Y-H, Le G-W (2013) Phytosterol supplementation improves antioxidant enzymes status and broiler meat quality, Pakistan. J Food Sci 23:163–171
Sañé E, Del Mondo A, Ambrosino L, Smerilli A, Sansone C, Brunet C (2021) The recent advanced in microalgal phytosterols: bioactive ingredients along with human-health driven potential applications. Food Rev Int 39(4):1859–1878. https://doi.org/10.1080/87559129.2021.1938115
Nazir M, Arif S, Khan RS, Nazir W, Khalid N, Maqsood S (2019) Opportunities and challenges for functional and medicinal beverages: current and future trends. Trends Food Sci Technol 88:513–526. https://doi.org/10.1016/j.tifs.2019.04.011
Poole J, Bentley J, Barraud L, Samish I, Dalkas G, Matheson A, Clegg P, Euston SR, Kauffman Johnson J, Haacke C (2020) Rising to the challenges: solution-based case studies highlighting innovation and evolution in reformulation. Nutr Bull 45:332–340
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This work was supported by the National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
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Rimsha Younas: investigation-equal, writing—original draft. Amna Sahar: conceptualization, writing—review and editing, supervision. Aysha Sameen: writing—review and editing. Muhammad Issa Khan: writing—review and editing. Muhammad Azhar Ali: writing—review and editing. Muhammad Arbaz Tahir, Muhammad Mohsin, and Muhammad Usman: writing—review and editing. Rana Muhammad Aadil: writing—review and editing, supervision.
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Younas, R., Sahar, A., Sameen, A. et al. A narrative review on extraction techniques of phytosterols and their applications in food industry. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-05007-w
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DOI: https://doi.org/10.1007/s13399-023-05007-w