Abstract
Arbutin, one of the main phenolic substances in pear fruits, has several health benefits. To optimize the extraction process of arbutin from pear fruitlets (‘Huangli No. 1’), ultrasonic-assisted extraction (UAE) was investigated as an efficient method. Single-factor experiments combined with response surface methodology (RSM) were used to determine the optimum sonication parameters. The results of single factor experiments showed that the maximum extraction yield of arbutin was obtained with 60% ethanol, 1:10 solid-to-liquid ratio, 20 min ultrasound time, and 648 W ultrasound power. After optimization by RSM, the optimal conditions were 70% ethanol, 1:12 solid-to-liquid ratio, 20 min ultrasound time, and 576 W ultrasound power. Under these conditions, the extraction yield of arbutin was 5309.27 ± 15.87 µg/g by UAE, which was significantly higher than that by conventional extraction (CE; 1935.25 ± 44.13 µg/g). UAE generated more cell damage in pear fruitlets than CE. Additionally, arbutin from UAE had a higher antioxidant activity than arbutin from CE. DPPH free radical scavenging activity, FRAP reducing power, and ABTS free radical scavenging activity were 22.25%, 27.72%, and 17.35% higher in UAE than in CE, respectively. UAE is an effective and rapid method for the extraction of arbutin from pear fruitlets.
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The research leading to these results received funding from the Research & Demonstration Project of Shanxi Functional Agriculture Common Key Technology under Grant Agreement No 201703D211011-04.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by TC, JL and FL. The first draft of the manuscript was written by TC and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Cui, T., Li, J., Li, N. et al. Optimization of ultrasonic-assisted extraction of arbutin from pear fruitlets using response surface methodology. Food Measure 16, 3130–3139 (2022). https://doi.org/10.1007/s11694-022-01412-3
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DOI: https://doi.org/10.1007/s11694-022-01412-3