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A Green, Efficient Approach on Extraction of Polyphenols from Fenugreek Seeds (Trigonella foenum-graecum): DES and β-Cyclodextrin Assisted Extraction

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Abstract

Trigonella foenum-graecum, is a widely used medicinal and culinary plant. The antioxidant compounds extracted from the seeds of this plant exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, antioxidant compounds from the seeds of Trigonella foenum-graecum were obtained using the deep eutectic solvent (DES) assisted and β-cyclodextrin assisted extraction method. The process was optimized by implementing a response surface methodology that took into consideration the following independent variables: β-cyclodextrin concentration (Cβ-CD) or DES concentration, solid-to-liquid ratio, and temperature. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging activity of Trigonella foenum-graecum antioxidant compounds on DPPH*, the total phenolic yield (YTP) and total flavonoid yield (YTF) was determined. Using the optimized conditions for DES extractions, the yields were 0.019 mmol TRE/g seed, 14.73 mg GAE/g seed and 0.092 mmol QE/g seed for AAR, YTP and YYF respectively. For β-cd assisted extraction, using the optimized conditions, the yields were 0.1213 mmol TRE/g seed, 12.89 mg GAE/g seed and 0.1602 mmol QE/g seed for AAR, YTP and YYF respectively. However, based on regression analysis and optimization based on solvent type it is observed that the extraction yield with β-cyclodextrin solutions is statistically significantly (P < 0.05) higher than the corresponding yield with solutions of deep eutectic solvent mixtures.

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Data Availability

The datasets generated during and/or analyzed during the current study are not publicly available due to possible patent application procedure but are available from the corresponding author on reasonable request.

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  1. Department of Food Science and Technology, Faculty of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Theofilos Frangopoulos

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Frangopoulos, T. A Green, Efficient Approach on Extraction of Polyphenols from Fenugreek Seeds (Trigonella foenum-graecum): DES and β-Cyclodextrin Assisted Extraction. Waste Biomass Valor 13, 4403–4415 (2022). https://doi.org/10.1007/s12649-022-01785-y

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