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Optimization of extraction process of total flavonoids from Cortex Lycii and its biological activities

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Abstract  

To research the optimization of ultrasonic-assisted extraction of total flavonoids from Cortex Lycii and its biological activities. Box-Behnken design experiment was used to optimize the extraction process, and AB-8 macroporous resin was used for purification. Its biological activities were studied by the inhibition rate of α-glucosidase and α- amylase and the scavenging rate of DPPH and ABTS. The optimal extraction parameters of total flavonoids from Cortex Lycii were as follows: the extraction temperature was 63 ℃, the ethanol volume fraction was 71%, the solid–liquid ratio was 28 mL/g, the extraction time was 61 min. Under this optimal condition, the yield of total flavonoids was 1.674 mg/g, which was consistent with the predicted value of 1.637 mg/g, and was 5.85 times higher than that of the traditional water extraction method. The inhibitory rate (IC50) of total flavonoids extract from Cortex Lycii on α-glucosidase was 3 μg/mL and on α-amylase was 5 μg/mL. The scavenging rate (IC50) of DPPH radical was 0.648 mg/mL and the scavenging rate (IC50) of ABTS radical IC50 was 0.012 mg/mL. The optimized extraction process with response surface design was reliable, which could be used for the extraction of total flavonoids from Cortex Lycii. It has strong hypoglycemic and antioxidant activities and has a good prospect for future utilization and development.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

This study was supported by the National Key Clinical Specialty Construction Project (Clinical Pharmacy) and the High-Level Clinical Key Specialty (Clinical Pharmacy) in Guangdong Province.

Funding

This research work was financially supported by the Guangdong Basic and Applied Basic Research Foundation (No. 2021A1515110565), the Medical Scientific Research Foundation of Guangdong Province of China (No. B2021051), the Scientific Research Project of Traditional Chinese Medicine Bureau of Guangdong Province of China (No. 20212119), the Science and Technology Program of Guangzhou, China (No. 202201010530), and the College Students’ innovation and entrepreneurship training program (No. 202210573026).

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Authors and Affiliations

  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Yao Wen, Xiang Zeng & Hongyu Tan

  2. Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems, Guangzhou, China

    Bing Liu

  3. Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China

    Bing Liu

Authors
  1. Yao Wen
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  2. Xiang Zeng
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  3. Hongyu Tan
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  4. Bing Liu
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Contributions

Methodology, YW and XZ; investigation, YW, XZ, and HT; formal analysis, YW, XZ, and HT; resources, YW; writing—original draft preparation, YW; writing—review and editing, Y W and XZ; supervision, YW and BL; project administration, YW and BL; funding acquisition, YW.

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Correspondence to Yao Wen or Bing Liu.

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Wen, Y., Zeng, X., Tan, H. et al. Optimization of extraction process of total flavonoids from Cortex Lycii and its biological activities. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03768-y

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