Abstract
Today’s green chemistry is pushing for the search and application of novel methods for the extraction of bioactive ingredients from biomass, which are directly related to human health such as food, pharmaceutics, and cosmetics. Therefore, the purpose of this study is to propose a green extraction method by application of automated solvent extraction from the leaves of Moringa oleifera. Central composite design together with response surface approach was exploited for designing of experimental work, modeling, and optimization reasons. Extraction time (40–60 min), particle size of the leaves (0.5–2 mm), and ethanol (solvent) concentration (30–90%, v/v) were the process factors, which were analyzed statistically by means of central composte design. The best conditions to attain the greatest total phenolic and flavonoid contents (34.201 mg-GAE and 162.349 mg-CE per g dried sample) are 60 min of extraction time, 1.25 mm of particle size and 87% (v/v) ethanol solution. While the most effective parameter in terms of total phenolic substance was extraction time, the most statistically significant variable in terms of flavonoids was solvent concentration. The generated second-order models were satisfactory, which was confirmed by validation study (< 2%). In order to verify the findings of antioxidant activity of the extracts, ABTS and DPPH assays were also conducted. A positive and satisfactory correlation (> 0.80) between test results confirms the accuracy of the results.
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Abbreviations
- FAO:
-
Food and Agriculture Organization
- WHO:
-
World Health Organization
- FDA:
-
Food and Drug Administration
- ASE:
-
Automated Solvent Extraction
- CCD:
-
Central Composite Design
- RSA:
-
Response Surface Approach
- TPC:
-
Total phenolic content
- TFC:
-
Total flavonoid content
- ABTS:
-
2.2'-Azino-bis (3-ethylbenzothiazoline)-6-sulfonic acid diammonium salt
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazil
- Trolox:
-
6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
- GAE:
-
Gallic acid equivalent
- DP:
-
Dried plant
- CE:
-
Catechin equivalent
- TEAC:
-
Trolox equivalent
References
Nerín C, Tovar L, Salafranca J (2008) Behaviour of a new antioxidant active film versus oxidizable model compounds. J Food Eng 84:313–320. https://doi.org/10.1016/j.jfoodeng.2007.05.027
Lourenço SC, Moldão-Martins M, Alves VD (2019) Antioxidants of natural plant origins: from sources to food industry applications. Molecules 24:4132
Đorđević V, Balanč B, Belščak-Cvitanović A et al (2014) Trends in encapsulation technologies for delivery of food bioactive compounds. Food Eng Rev 7:452–490
Ayala-Zavala JF, Vega-Vega V, Rosas-Domínguez C et al (2011) Agro-industrial potential of exotic fruit byproducts as a source of food additives. Food Res Int 44:1866–1874
Kim MJ, Moon Y, Tou JC et al (2016) Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.). J Food Compos Anal 49:19–34
Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Fernández-Gutiérrez A (2010) Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules 15:8813–8826. https://doi.org/10.3390/molecules15128813
Chemat F, Vian MA, Cravotto G (2012) Green extraction of natural products: concept and principles. Int J Mol Sci 13:8615–8627. https://doi.org/10.3390/ijms13078615
Wu L, Li L, Chen S et al (2020) Deep eutectic solvent-based ultrasonic-assisted extraction of phenolic compounds from Moringa oleifera L. leaves: optimization, comparison and antioxidant activity. Sep Purif Technol 247:117014. https://doi.org/10.1016/j.seppur.2020.117014
Rodríguez GM, Sibaja JC, Espitia PJP, Otoni CG (2020) Antioxidant active packaging based on papaya edible films incorporated with Moringa oleifera and ascorbic acid for food preservation. Food Hydrocoll 103:105630. https://doi.org/10.1016/j.foodhyd.2019.105630
Albarri R, Toprakçı İ, Kurtulbaş E, Şahin S (2021) Estimation of diffusion and mass transfer coefficients for the microwave-assisted extraction of bioactive substances from Moringa oleifera leaves. Biomass Convers Biorefinery 1–8.https://doi.org/10.1007/s13399-021-01443-8
Albarri R (2021) Şahin S (2021) Kinetics, thermodynamics, and mass transfer mechanism of the ultrasound-assisted extraction of bioactive molecules from Moringa oleifera leaves. Biomass Convers Biorefinery 1:1–8. https://doi.org/10.1007/S13399-021-01686-5
Vongsak B, Sithisarn P, Mangmool S et al (2013) Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind Crops Prod 44:566–571. https://doi.org/10.1016/J.INDCROP.2012.09.021
Nobossé P, Fombang EN, Mbofung CMF (2018) Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves. Food Sci Nutr 6:2188–2198. https://doi.org/10.1002/FSN3.783
Wang Y, Gao Y, Ding H et al (2017) Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity. Food Chem 218:152–158. https://doi.org/10.1016/J.FOODCHEM.2016.09.058
Bozinou E, Karageorgou I, Batra G et al (2019) Pulsed electric field extraction and antioxidant activity determination of moringa oleifera dry leaves: a comparative study with other extraction techniques. Beverages 5:8. https://doi.org/10.3390/BEVERAGES5010008
Pollini L, Tringaniello C, Ianni F et al (2020) Impact of ultrasound extraction parameters on the antioxidant properties of Moringa Oleifera leaves. Antioxidants 9:277. https://doi.org/10.3390/ANTIOX9040277
Lin X, Wu L, Wang X et al (2021) Ultrasonic-assisted extraction for flavonoid compounds content and antioxidant activities of India Moringa oleifera L. leaves: simultaneous optimization, HPLC characterization and comparison with other methods. J Appl Res Med Aromat Plants 20:100284. https://doi.org/10.1016/J.JARMAP.2020.100284
Chemat F, Abert-Vian M, Fabiano-Tixier AS et al (2019) Green extraction of natural products. Origins, current status, and future challenges. TrAC - Trends Anal Chem 118:248–263
Moradi M, Fazlzadehdavil M, Pirsaheb M et al (2016) Response surface methodology (RSM) and its application for optimization of ammonium ions removal from aqueous solutions by pumice as a natural and low cost adsorbent. Arch Environ Prot 42:33–43. https://doi.org/10.1515/aep-2016-0018
Sreelatha S (2009) Padma PR (2009) Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods Hum Nutr 644(64):303–311. https://doi.org/10.1007/S11130-009-0141-0
Jahan S, Abdulkadir AR, Zawawi D, Jahan MS (2015) DPPH antioxidant activity, total phenolic and total flavonoid content of different part of Drumstic tree (Moringa oleifera Lam.) DPPH antioxidant activity, total phenolic and total flavonoid content of different part of Drumstic tree (Moringa oleifera Lam.). Available online www.jocpr.com. J Chem Pharm Res 7:1423–1428
Floegel A, Kim DO, Chung SJ et al (2011) Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal 24:1043–1048. https://doi.org/10.1016/J.JFCA.2011.01.008
Martysiak-Zurowska D, Wenta W (2012) A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Acta Sci Pol Technol Aliment 11:83–89
Sridhar K, Charles AL (2019) In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: estimation methods for EC50 using advanced statistical programs. Food Chem 275:41–49. https://doi.org/10.1016/J.FOODCHEM.2018.09.040
Liu HL, Chiou YR (2005) Optimal decolorization efficiency of Reactive Red 239 by UV/TiO2 photocatalytic process coupled with response surface methodology. Chem Eng J 112:173–179. https://doi.org/10.1016/J.CEJ.2005.07.012
Plazzotta S, Ibarz R, Manzocco L, Martín-Belloso O (2020) Optimizing the antioxidant biocompound recovery from peach waste extraction assisted by ultrasounds or microwaves. Ultrason Sonochem 63:104954. https://doi.org/10.1016/j.ultsonch.2019.104954
Yang L, Jiang J-G, Li W-F et al (2009) Optimum extraction process of polyphenols from the bark of Phyllanthus emblica L. based on the response surface methodology. J Sep Sci 32:1437–1444. https://doi.org/10.1002/jssc.200800744
Lapornik B, Prošek M, Wondra AG (2005) Comparison of extracts prepared from plant by-products using different solvents and extraction time. J Food Eng 71:214–222. https://doi.org/10.1016/j.jfoodeng.2004.10.036
Zhang Z, Pang X, Ji Z, Jiang Y (2001) Role of anthocyanin degradation in litchi pericarp browning. Food Chem 75:217–221. https://doi.org/10.1016/S0308-8146(01)00202-3
Chen M, Zhao Y, Yu S (2015) Optimisation of ultrasonic-assisted extraction of phenolic compounds, antioxidants, and anthocyanins from sugar beet molasses. Food Chem 172:543–550. https://doi.org/10.1016/j.foodchem.2014.09.110
Baldosano HY, Beatriz Micaela Castillo MG, Danica Elloran CH, Bacani FT (2015) Presented at the DLSU Research Congress. 3
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Raneen Albarri: Software, Validation, Formal analysis Selin Şahin: Conceptualization, Methodology, Writing- Reviewing and Editing.
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Albarri, R., Şahin, S. A green method for the extraction of Moringa oleifera leaves: evaluation of several in vitro assays for bioactive properties. Biomass Conv. Bioref. 14, 6397–6405 (2024). https://doi.org/10.1007/s13399-022-02690-z
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DOI: https://doi.org/10.1007/s13399-022-02690-z