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Optimization of extraction process of polyphenols from wild halotolerant cyanobacteria, Phormidium versicolor (NCC 466)

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Abstract

This study focuses on the extraction optimization and characterization of polyphenols from the wild blue-green microalga Phormidium versicolor, as well as in vitro evaluation of antioxidant and antimicrobial activities. The optimal extraction conditions were as follows: extraction temperature at 52 °C, extraction time of 30 min, and solid-to-liquid ratio of 1:50 g mL−1 under ultrasonic-assisted extraction in the ethanol-based solvent. Yield of the polyphenol extract of Phormidium versicolor (P-Pv) increased from 40.35 to 76.74 mg g−1 dw. Polyphenol compounds of P-Pv were analyzed through high-performance liquid chromatography and Fourier transform infrared (FTIR) spectroscopy. Results revealed the presence of four polyphenol compounds with the highest concentration of kaempferol (31.8 μg mg−1) extract. The FTIR analysis shows the existence of alcohol bond and flavonoids. In vitro antioxidant activity suggested that P. versicolor polyphenol extract had effective scavenging activity of DPPH, ABTS, and nitric oxide radicals. Furthermore, the current extract exhibited effective antimicrobial properties in vitro. Such pharmaceutical activities could prove valuable for potential future applications involving the P. versicolor cyanobacteria.

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Contributions

I. D. B. conceived the study, executed microalgae cultures, prepared materials, performed the statistical analysis, analyzed extracts, and wrote the manuscript. D. B. participated in the study design, provided technical advices, and reviewed the manuscript. H. A. provided academic advices. All authors read and approved the final manuscript.

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Correspondence to Ines Dahmen-Ben Moussa.

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Dahmen-Ben Moussa, I., Belhaj, D. & Ayadi, H. Optimization of extraction process of polyphenols from wild halotolerant cyanobacteria, Phormidium versicolor (NCC 466). Biomass Conv. Bioref. 14, 3397–3407 (2024). https://doi.org/10.1007/s13399-022-02651-6

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