NADES assisted integrated biorefinery concept for pectin recovery from kinnow (Citrus reticulate) peel and strategic conversion of residual biomass to L(+) lactic acid

The present study aims to establish an integrated strategy for valorization of kinnow peel waste. A total of ten natural deep eutectic solvents (NADESs) were exploited for extraction of pectin. The highest yield of pectin enriched material was reported 35.66 % w/dw using choline chloride-Maltose based NADES. The extraction process parameters and chemical composition of NADES influenced the yield and different associated physico-chemical attributes of the pectin enriched material. All the recovered pectin enriched materials found to be composed of low methoxy pectin (degree of methylation: 18.41-40.26 %) and galacturonic acid (GalA) content was in range of 67.56-78.22 %. The Principal Component Analysis (PCA) was used to categorise isolated pectin enriched materials based on similarities and differences. The liquid fraction upon pectin extraction presented a considerable amount of fermentable sugar which was further utilized for lactic acid production by microbial intervention. The microbial strain Lactobacillus amylophilus GV6 was exploited for lactic acid fermentation where the highest yield reached 55.59 g/L. A sustainable and straight-forward biorefinery concept was developed for extraction of pectin enriched material and lactic acid production from kinnow peel waste with potential application in food and biotechnological sectors.