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Extraction of flavonoids and phenolics from Berberis kongboensis fruit

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Abstract

Response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) and total phenols (TP) from Berberis kongboensis fruit. Single-factor experiments were conducted with the ultrasonication power, liquid–solid ratio, ethanol concentration, extraction temperature, and extraction time. A Plackett–Burman design was used to identify the main factors affecting the extraction rates of TF and TP. A Box–Behnken design was used to further optimize the ethanol concentration, solvent/sample ratio, and extraction temperature. Under the optimum conditions, the ethanol concentration of 34.67%, solvent/sample ratio of 38.44 mL/g, and extraction temperature of 46.53 °C, the yields of TF and TP were 107.92 mg rutin equivalents/g and 4.86 mg gallic acid equivalents/g, respectively. The extract from UAE had a better yield and higher antioxidant activities in vitro than that obtained by conventional solvent extraction. The B. kongboensis fruit is rich in flavonoids and phenolics, and it has potential application value in the food industry.

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Data Availability

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Abbreviations

UAE:

Ultrasound-assisted extraction

CSE:

Conventional solvent extraction

TF:

Total flavonoids

TP:

Total phenolics

RT:

Rutin

GA:

Gallic acid

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

FRAP:

Ferric ion reducing antioxidant power

TRAP:

Total radical-trapping antioxidant parameter

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Acknowledgements

This work was supported by the Natural Science Foundation of Heilongjiang Province (Grant No. LH2020C036), the Forestry Science Discipline Innovation Team Construction Project of Tibet Agriculture & Animal Husbandry University (Grant No. ZangCaiYuZhi 2020-001), the Central Government Guidance Local Regional Innovation Systems Construction Program (Grant No. XZ202201YD0025C), and the 111 Project (Grant No. B20088). We thank Gabrielle David, PhD, for editing the English text of a draft of this manuscript.

Funding

Natural Science Foundation of Heilongjiang Province (Grant No. LH2020C036).

Forestry Science Discipline Innovation Team Construction Project of Tibet Agriculture & Animal Husbandry University (Grant No. ZangCaiYuZhi 2020-001).

Central Government Guidance Local Regional Innovation Systems Construction Program (Grant No. XZ202201YD0025C).

111 Project (Grant No. B20088).

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Authors and Affiliations

  1. College of Resources and Environment, Tibet Agriculture & Animal Husbandry University, Nyingchi, 860000, People’s Republic of China

    Xiaoqiang Chen & Hong Yang

  2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Xiaoqiang Chen, Shihan Yang, Jinshan Zhang, Yuyuan Huang & Ying Zhang

  3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Xiaoqiang Chen, Shihan Yang, Jinshan Zhang, Yuyuan Huang & Ying Zhang

  4. Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Xiaoqiang Chen & Ying Zhang

  5. Heilongjiang Provincial Key Laboratory of ecological utilization of Forestry-based active substances, Harbin, 150040, People’s Republic of China

    Xiaoqiang Chen & Ying Zhang

  6. National Engineering Laboratory of BioResource EcoUtilization, Harbin, 150040, People’s Republic of China

    Xiaoqiang Chen & Ying Zhang

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  1. Xiaoqiang Chen
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Contributions

Xiaoqiang Chen: conceptualization, data curation, formal analysis, investigation, methodology, visualization, project administration, and writing—original draft

Shihan Yang: formal analysis, methodology, resources, and writing—review and editing. Hong Yang: conceptualization, methodology, Resources, Supervision, Funding acquisition. Jinshan Zhang: data curation and methodology. Yuyuan Huang: visualization and writing—review and editing. Ying Zhang: conceptualization, data curation, methodology, writing—review and editing, supervision, and funding acquisition

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Correspondence to Ying Zhang.

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Chen, X., Yang, S., Yang, H. et al. Extraction of flavonoids and phenolics from Berberis kongboensis fruit. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03906-6

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