Abstract
Lack of physical exercise; unhealthy eating habits; exposure to environmental pollutants, heavy metals, and food additives; and modern life style lead to oxidative stress. Increased production of reactive oxygen species (ROS) damage body cells and tissues and contribute to more risk of chronic diseases like cancer, diabetes, and cardiovascular impairments. Oxidative damage and chronic diseases can be prevented through the use of effective antioxidants. Moringa oleifera, a food plant, has potential as antioxidant, antimicrobial agent, anti-inflammatory, and antidiabetic. The current investigation was planned to extract bioactive compounds from Moringa oleifera leaf powder (MoLP). Moringa leaves were collected, dried, and ground, and the aqueous and ethanolic extracts of MoLP were prepared by maceration and then characterized for phytochemical profiling by HPLC and Fourier transform infrared (FTIR) spectroscopy. HPLC of Moringa oleifera leaf extract (MoLE) revealed that 70% extract contain more concentrations of quercetin (45.01 ppm), gallic acid (3.26 ppm), chlorigenic acid (8.45 ppm), p-coumaric acid (1.38 ppm), ferulic acid (5.82 ppm), and sinapic acid (2.64 ppm). FTIR spectroscopy was performed to investigate the functional group profile of Moringa oleifera leaf extract. The FTIR analysis identified the presence of major functional groups including alcohols, alkanes, alkenes, nitro compounds, ether, ester, carboxylic acid, aromatic, aliphatic bromo compounds, aryl disulfide, isocyanates, and phenols. The research shows that the phytochemicals in Moringa oleifera act as anti-oxidant to stabilize free radicals and recommended to be used in human diet as food supplements.
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Acknowledgements
Thanks to GAIN (Axencia Galega de Innovación) for supporting this research (grant number IN607A2019/01).
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S.K.: conceptualization, methodology, investigation, validation, formal analysis, writing-original draft preparation. M.A.: methodology, investigation, validation, formal analysis, contribution in writing. S.M.: methodology, investigation, validation, formal analysis, and contribution in writing in relevant section. F.S.: methodology, investigation, validation, formal analysis, and contribution in writing in relevant section. M.M.A.N.R.: conceptualization, methodology, investigation, validation, formal analysis, writing—original draft preparation. U.R.: review and editing, final draft supervision, and monitoring. J.M.L.: review and editing, final draft supervision, and monitoring. All authors read and approved the final manuscript.
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Shakeela Khalid declares that she has no conflict of interest. Muhammad Arshad declares that he has no conflict of interest. Shahid Mahmood declares that he has no conflict of interest. Farzana Siddque declares that she has no conflict of interest. Ume Roobab declares that she has no conflict of interest. Muhammad Modassar Ali Nawaz Ranjha declares that he has no conflict of interest. Jose M. Lorenzo declares that he has no conflict of interest.
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Khalid, S., Arshad, M., Mahmood, S. et al. Extraction and Quantification of Moringa oleifera Leaf Powder Extracts by HPLC and FTIR. Food Anal. Methods 16, 787–797 (2023). https://doi.org/10.1007/s12161-023-02470-z
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DOI: https://doi.org/10.1007/s12161-023-02470-z