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emulatebio.com
In 2025, momentum
around New Approach Methodologies (NAMs) shifted decisively from
advocacy to action. Regulatory agencies across the globe took
concrete steps to reduce reliance on animal testing and actively
[…] The post 2025: A Pivotal Year for NAMs and the Top 10
Organ-Chip Publications Supporting Their Adoption appeared first on
Emulate .
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ncbi.nlm.nih.gov
The Food and Drug
Administration recently announced a plan to phase out animal-testing
requirements for drug assessments based on advances in new approach
methodologies, including human organ-on-a-chip (Organ Chip)
microfluidic culture technology. Although Organ Chips are being
explored in many [...]
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biospace.com
[...] U.S.
regulators in April announced a seismic shift in their approach to
preclinical in vivo testing. In a decision with immediate and
potentially far-reaching implications for drug development, the FDA
stated it would phase outin vivo testing requirements fo [...]
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hitconsultant.net
What You Should Know
– Cedars-Sinai has secured a $5.05M ARPA-H contract to build
KronosRx, an AI platform that predicts drug toxicity by pairing
millions of electronic health records with “patient
avatars”—human stem-cell-derived organoids. – The strategic
initiative aims to solve the 30% failure rate of clinical trials
caused by adverse drug reactions that animal ... Read More
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koreabiomed.com
MEPSGEN, a company
specializing in microphysiological systems (MPS) and drug delivery
systems (DDS) platforms, announced Wednesday that it has joined as a
technology partner in the newly established Centre for Intestinal
Systems (CIS) at Imperial College London.The CIS is a research hub
specializing
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news-medical.net
Researchers at the
Francis Crick Institute and AlveoliX have developed the first human
'lung-on-chip' model using stem cells taken from only one
person.
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cordis.europa.eu
L'entreprise
spin-off Syntopia met à profit les résultats du projet SYNEBIO en
créant une plateforme microfluidique révolutionnaire qui peut
accélérer la découverte de nouveaux médicaments.
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technologynetworks.com
KAIST researchers
developed a modular organ-on-a-chip that reproduces rhabdomyolysis,
revealing how drug-induced muscle damage releases toxins that
progressively cause acute kidney injury.
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hal.science
Face aux limitations
soulevées par l'utilisation d'animaux à des fins de recherche sur
le plan éthique, économique et de pertinence du modèle animal pour
une extrapolation à l'Homme, il est nécessaire de se tourner vers
des méthodes alternatives. Les études toxicologiques et
réglementaires sont particulièrement concernées par ce besoin de
méthodes alternatives étant donné que ces
|
|
biospace.com
[...] Ainnocence
today
announced a major milestone in artificial-intelligence–driven drug
discovery in
the white
paper by C&EN: a sequence-first AI platform capable of screening
billions of small-molecule and antibody candidates across the entire
genome in
hours o [...]
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hal.science
Journal of
International Research in Medical and Pharmaceutical Sciences, 2026,
21 (1), pp.26 - 45
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usinenouvelle.com
Depuis plusieurs
années, la médecine évolue vers un nouveau paradigme : la
médecine personnalisée. Loin de la logique d'un traitement pour
tous, celle-ci cherche à adapter la stratégie thérapeutique aux
caractéristiques de chaque patient.
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news-medical.net
Most cells in the
human body exist in complex three-dimensional environments, yet they
are still commonly studied on flat plastic dishes.
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laas.hal.science
[...]
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joint-research-centre.ec.europa.eu
[...] The Biomedical
models Hub (BimmoH) is the world largest dataset containing
scientific article references making use of human biology-based
models. Powered by AI, it consolidates information on a wide range of
models based on human biology, including organ-on-a-chi [...]
|
|
sciencepost.fr
Le compte à rebours
est lancé pour Artemis II. Pour la première fois depuis 54 ans, des
êtres humains vont quitter l’orbite terrestre basse pour
s’aventurer plus loin dans l’espace que n’importe qui avant
eux. Mais ce que la NASA met moins en avant que les visages
héroïques de l’équipage, c’est la cargaison scientifique
fascinante […]
|
|
biospace.com
[...] The
Blood-Brain Barrier (BBB) is the body's most formidable defense
system, designed to protect brain function, but it is also the
greatest obstacle to treating neurological diseases. Approximately
98% of small-molecule drugs and nearly all large-molecule biologic
[...]
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|
publications.jrc.ec.europa.eu
The 5th edition of
the JRC Summer School took place in May 2025, tailored for
post-graduate students and early-career scientists working in
bioscience research and focusing on non-animal methods and
technologies.
|
|
antidote-europe.eu
Avant de se prêter
à un essai de nouveau médicament, tout patient ou volontaire sain
doit donner son accord par écrit. Il est censé avoir reçu des
informations sur l'objectif de l'essai et ses possibles risques. Dans
quelle mesure ceux-ci sont-ils connus et correctement évalués ?
Antidote Europe a creusé cette question, avec le concours… Lire la
suite
|
|
3dprint.com
We’re starting
with business news in today’s 3D Printing News Briefs, as AMGTA
announced EOS as a new Principal Member, and the company’s Head of
Sustainability as the newest member...
|
|
cordis.europa.eu
NanoFlash aims to
develop disruptive nano-enabled technology to restore damaged
neuronal networks through precise stimulation of specific retinal
cells. Focusing on non-genetic reactivation of physiological vision,
NanoFlash targets treatment of retinitis pigments (RP) and...
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|
globenewswire.com
Research-use-only
offerings are commercially available, enabling immediate product and
service revenue generation
|
|
lejournaldesentreprises.com
[...] Diagnoly lève
5,5 millions d’euros pour déployer son copilote d’échographie
prénatale par IA
Ivan Voznyuk (PDG et cofondateur), Camille Dupont
(CTO) et le Dr Edwin Quarello (directeur médical et cofondateur) de
Diagnoly [...]
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prnewswire.com
[...] LONDON, Jan.
9, 2026 /PRNewswire/ -- M&G Investments (M&G) today announces that it
has led a US$50 million Series C funding round for bit.bio, the
Cambridge-based biotech company pioneering next-generation human cell
programming technology. The investment of patien [...]
|
|
prnewswire.com
Additional funding
of $20 million accelerates commercialization of Trailhead's
iPSC-derived human cell and media products. BEACHWOOD, Ohio and
CAMBRIDGE, Mass., Jan. 12, 2026 /PRNewswire/ -- Trailhead Biosystems
Inc. (www.trailbio.com), a biotechnology company advancing human
cell...
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prnewswire.com
BOSTON and SEOUL,
South Korea, Jan. 5, 2026 /PRNewswire/ -- Xellar Biosystems
("Xellar"), a globally leading innovator in next-generation
artificial intelligence- and computer vision-assisted,
organ-on-a-chip-based drug discovery and development platforms, today
announced that it has...
|
|
eu-startups.com
Geneva-based BioTech
startup FluoSphera today announced that it has raised €1.23 million
(CHF 1.15 million) in funding to make drug discovery faster, safer,
and more human-relevant with its patented multi-tissue platform.
The round was led by Soulmates Ventures and a Swiss business angel,
with participation from IndieBio New York. The startup will use the
funding […] The post Female-led
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euroocs.eu
?? Join Us at the
EUROoCS Digital Lecture! ?? We are delighted to announce that Prof.
Milica Radisic, Professor at the University of Toronto, Tier 1 Canada
Research Chair in Organ-on-a-Chip Engineering, and Senior Scientist
at University Health Network, will be the next speaker in the EUROoCS
Digital Lecture Series. ?? Talk Title: "Self-Driving Laboratory
… Continue reading "EUROoCS Digital
|
|
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publications.jrc.ec.europa.eu
The 5th edition of
the JRC Summer School took place in May 2025, tailored for
post-graduate students and early-career scientists working in
bioscience research and focusing on non-animal methods and
technologies.
|
|
ateliersinserm.dakini-pco.com
The aim of this
2-step workshop is first to provide to the French scientific
community an overview of the latest advances in the field of
organ-on-chip, from general introduction to recent advance-ments
regarding pathophysiology research (metabolic processes /neuroscience
/cancer/infection/immunity) including biosensors integration.
|
|
euroocs.eu
?? Join us for the
next EUROoCS Digital Lectures - Young Investigators Edition, taking
place on February 17th from 17:00-18:00 CET. ?? We're excited to
welcome three outstanding early-career researchers who are advancing
organ-on-chip, biofabrication, and sensing technologies: ?? M.
Cagatay Karakan Postdoctoral researcher in Prof. Christopher Chen's
lab at Boston University and the Wyss …
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euroocs.eu
We are pleased to
share that the application for the EUROoCS Summerschool 2026 is open!
Find all information here: Organ-on-Chip Summer School 2026 –
EUROoCS Successful applicants will be notified by 24 April 2026! The
post Application EUROoCS Summerschool is open! appeared first on
EUROoCS .
|
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worldwide.espacenet.com
The present
invention relates to organoid co-cultures and their use in the
investigation of diseases.
|
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worldwide.espacenet.com
No abstract
available
|
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worldwide.espacenet.com
Disclosed herein are
hepato-biliary-pancreatic organoid ("HBPO" or "EBP organoid")
compositions, and methods of making and using
hepato-biliary-pancreatic organoid compositions. The disclosed
compositions may have two or more functions selected from hepatic
tissue function, biliary tissue function, exocrine pancreatic
function, and endocrine pancreatic tissue function. Methods of
treating
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worldwide.espacenet.com
The present
invention refers to the biomedical field. Particularly, the present
invention refers to an in vitro method for obtaining a cortical brain
organoid, a cortical brain organoid obtained or obtainable by said
method and its application as a medicament, preferably in a method of
treating a brain disorder, condition, or disease.
|
|
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worldwide.espacenet.com
No abstract
available
|
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worldwide.espacenet.com
No abstract
available
|
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worldwide.espacenet.com
The present
invention relates to a technology for constructing and culturing hair
follicle organoids for hair drug screening methods and production of
hair transplant materials. More specifically, in the present
invention, hair follicles were isolated from existing skin organoids
derived from human induced pluripotent stem cells and cultured on
collagen- and Matrigel-coated culture inserts to
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|
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worldwide.espacenet.com
Provided is a
composition for obtaining and/or culturing the human normal tissue
derived organoids or the human tumor tissue derived organoids, the
composition includes an ALK inhibitor (e.g., LDN214117), a WNT
agonist (e.g., CHIR), an EGF agonist (e.g., EGF) and an FGF agonist
(e.g., FGF2), and optionally an IKKε inhibitor.
|
|
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worldwide.espacenet.com
An assay plate
system for assaying a cell culture model, such as an organoid,
comprises an assay plate, an upper surface, a grid array of wells
comprising at least two rows of wells, each row comprising at least
two wells, each well having an open top formed in the upper surface
and a closed transparent base, and at least two columns of wells,
each column comprising at least three wells, each
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|
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worldwide.espacenet.com
Automating
operations and handling of mesoscale biological objects that range in
size from ̃100 μm to ̃1 mm can accelerate the advancement of
disease modeling and drug discovery based on organoids and
cell-carrier systems. The device leverages flow driven by rotating
shafts in a Stokes flow regime to develop a gentle robotic
manipulation system for objects based on controllable arrays of
|
|
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worldwide.espacenet.com
The present
disclosure provides chimeric surfaces. The chimeric surfaces may be
thin films. In various embodiments, the thin films may be disposed on
a substrate. Articles of manufacture may have one or more chimeric
surfaces. Also disclosed are methods of making organoids from cancer
cells and methods of determining whether a subject has cancer. The
thin films may be formed from one or more
|
|
|
worldwide.espacenet.com
In vitro process for
producing organoids from mammalian cells, the process comprising or
the steps of cultivating pluripotent stem cells (PSC) in cell culture
medium in a stirred tank bioreactor under conditions suitable for
producing PSC aggregates, removing PSC aggregates suspended in cell
culture medium from the bioreactor, and preferably in a flow channel,
encapsulating PSC aggregates
|
|
|
worldwide.espacenet.com
Disclosed herein are
hepato-biliary-pancreatic organoid ("HBPO" or "HBP organoid")
compositions, and methods of making and using
hepato-biliary-pancreatic organoid compositions. The disclosed
compositions may have two or more functions selected from hepatic
tissue function, biliary tissue function, exocrine pancreatic
function, and endocrine pancreatic tissue function. Methods of
treating
|
|
|
worldwide.espacenet.com
Provided are: a
therapeutic agent for NK2 homeobox 1 (NKX2-1)-negative lung
adenocarcinoma containing a Porcupine inhibitor as an active
component; a NKX2-1-negative lung adenocarcinoma organoid; and a
method for screening for a therapeutic agent for NKX2-1-negative lung
adenocarcinoma, the method including culturing an NKX2-1-negative
lung adenocarcinoma organoid in the presence of a test
|
RH : offres d'emploi, thèses, postocs
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pepr-medooc.fr
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ceva.com
.
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etap-lab.com
.
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spartha-medical.welcomekit.co
.
|
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alveolelab.com
.
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groupeicare.com
.
|
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welcometothejungle.com
.
|
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cherrybiotech.com
.
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netri.com
.
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gdrmicrofluidique.com
.
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gdr-organoides.cnrs.fr
.
|
Publications organes sur puce
|
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ncbi.nlm.nih.gov
Tumor stem cells
(TSCs) play a pivotal role in the development of tumor organoids.
Consequently, the development of effective methods for the isolation
and differential induction of TSCs is essential for the establishment
of tumor organoids. In this study, we [...]
|
|
|
ncbi.nlm.nih.gov
Venous return
depends on compliant vessels, competent valves, and auxiliary pumps
such as the calf and thoracic muscles. Disruption of these
mechanisms, through immobility, valve dysfunction, or inflammation,
creates disturbed flow and heterogeneous shear that drive venous
dysfunction and disease [...]
|
|
|
ncbi.nlm.nih.gov
Dissolution dynamic
nuclear polarization NMR Spectroscopy (dDNP-NMR) has become a
transformative tool for metabolic studies by significantly enhancing
signal sensitivity more than 3 orders of magnitude compared to
traditional NMR. However, NMR detection probes are optimized for
round narrow glass [...]
|
|
|
ncbi.nlm.nih.gov
Reliable prediction
of reproductive toxicity remains a critical challenge in drug
development and environmental safety. Here, a biomarker-integrated,
fluorescent reporter-based reproductive organ-on-a-chip platform that
recapitulates the multicellular composition, 3D architecture,
endocrine signaling, and cyclic dynamics of the human menstrual
cycle, [...]
|
|
|
onlinelibrary.wiley.com
Organoids and
organ‐on‐a‐chips are advancing reproductive system research. In
the female reproductive system, applications include cancer organoid
models, placental chips, and hormone simulation models. For the male
reproductive system, research focuses on drug resistance mechanisms,
co‐culture platforms, and infertility studies. These refined in
vitro models enhance our understanding of
|
|
|
ncbi.nlm.nih.gov
Periodontitis (PD)
is a chronic inflammatory disease driven by a dysbiotic oral biofilm,
leading to the progressive destruction of the tooth-supporting
tissues. Peri-implantitis (PI) involves a similar plaque-induced
inflammatory process that results in the loss of bone around dental
implants. [...]
|
|
|
ncbi.nlm.nih.gov
The fabrication of
physiologically relevant microvascular networks remains a major
challenge in Organ-on-a-chip (OoC) technologies, largely due the
dependence on plastic materials and the generation of channels with
non-physiological, rectangular cross-sections. Here, we present a
novel approach for the in-situ [...]
|
|
|
ncbi.nlm.nih.gov
The Food and Drug
Administration recently announced a plan to phase out animal-testing
requirements for drug assessments based on advances in new approach
methodologies, including human organ-on-a-chip (Organ Chip)
microfluidic culture technology. Although Organ Chips are being
explored in many [...]
|
|
|
ncbi.nlm.nih.gov
This study aimed to
identify the key determinants of vascular networks (VN) morphology,
with a particular focus on the role of endothelial cell body area
expansion. We introduced a quantitative metric, the cell body area
expansion (CBE) score, to assess [...]
|
|
|
onlinelibrary.wiley.com
We developed a
“Buildable and Linkable Organ on a Chip” (BLOC) that can
construct diverse microphysiological systems (MPSs). The BLOC is
standardized to the same size and has one of the functions of
“Culture,” “Control,” or “Analysis.” Users can freely
configure various MPSs, including developing perfusion, cytotoxicity
analysis, and biochemical analysis devices, by connecting
|
|
|
onlinelibrary.wiley.com
The
Synovium‐on‐Chip comprises a thin microporous PDMS membrane to
support co‐culture of fibroblast‐like synoviocytes (FLS),
THP‐1‐derived macrophages, and endothelial cells, enabling
real‐time analysis of synovial‐vascular interactions. FLS
migration through the pores drives endothelial remodeling, while
TNF‐α stimulation induces robust inflammatory activation,
providing a
|
|
|
ncbi.nlm.nih.gov
Organ-on-a-chip
(OoC), as a highly biomimetic microphysiological system, is
demonstrating significant potential in oral medicine research and
clinical application. This review outlines the latest advances and
specific applications of OoC in the field, with a focus on its value
in [...]
|
|
|
ncbi.nlm.nih.gov
Pancreatic ductal
adenocarcinoma (PDAC) is a fatal malignancy. Current conventional
chemotherapeutics are inadequate in controlling the disease; hence,
there is an urgent need for precision medicine. Ex vivo models that
replicate the tumor and its microenvironment can advance precision
medicine [...]
|
|
|
hal.science
Journal of
International Research in Medical and Pharmaceutical Sciences, 2026,
21 (1), pp.26 - 45
|
|
|
ncbi.nlm.nih.gov
Organ-on-a-chip is
highly valuable for tumor drug screening, but further exploration in
gastric cancer is needed. Here, we propose an innovative platform to
cultivate primary gastric cancer cells using microfluidic
encapsulation, enabling three-dimensional (3D) tumor growth and
clinical drug assessment. [...]
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mdpi.com
The FDA plans to
gradually replace animal testing with organoid and organ-on-a-chip
technologies for drug safety assessment, driving surging demand for
gut-on-a-chip in food and drug safety evaluation and highlighting the
need for efficient, precise chip designs. Oxygen gradients are
central to these devices because they shape epithelial metabolism,
microbial co-culture, and overall gut
|
|
|
ncbi.nlm.nih.gov
The lymphatic
system-integral to fluid balance, immune surveillance, and lipid
absorption-is frequently overlooked despite its vital roles.
Traditional research modalities, including static two-dimensional
cultures and animal models, have illuminated key molecular and
cellular features but fall short in recapitulating human [...]
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scopus.com
PublicationName:
IscienceAffiliations: University of Arizona College of Medicine –
Phoenix / United States
doi: 10.1016/j.isci.2025.114236
eIssn: 25890042
Volume: 29
IssueIdentifier: 1
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link.springer.com
The blood–brain
barrier (BBB) and blood-cerebrospinal fluid barrier (BCSFB) are
critical interfaces that protect the central nervous system (CNS)
from the surveilling immune system in the body. However, they also
pose challenges for drug delivery and disease modeling. Over the past
decade, organ-on-a-chip technology has revolutionized in vitro
modeling of these barriers by incorporating
|
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biomedcentral.com
Background Cancer is
the second leading cause of death worldwide. While significant
progress has been made in early detection and treatment, metastasis
remains the major cause of cancer-related morbidity and mortality. In
the last decade the rate of long-term survivorship of metastatic
cancer has continued to improve and overcoming resistance to therapy
has now become a challenge. Developing
|
|
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nature.com
HepG2 cells were
cultured into liver organoids (more native liver-like than 2D
cultures) and integrated into a microfluidic chip, which regulates
flow, nutrients, and waste to simulate in vivo hepatic
microcirculation. Key findings: Cadmium (Cd) exposure shows a
positive dose-response with liver damage; 40 ng/mL selenium (Se)
alleviates 3 sets of liver biochemical dysfunctions under
40 μg/mL
|
|
|
ncbi.nlm.nih.gov
The endometrium is
the uterine lining that supports implantation and pregnancy. Existing
in vitro systems only partly capture epithelial structure and
function. We built a microfluidic model of the human endometrial
epithelium using patient-derived organoids and defined a
parameterized device [...]
|
|
|
ncbi.nlm.nih.gov
Organoid research
offers valuable insights into human biology and disease, but
reproducibility and scalability remain significant challenges,
particularly for epithelial organoids. Here, we present an integrated
microfluidic platform that addresses these limitations by enabling
high-throughput generation of uniform hydrogel microparticles
[...]
|
|
|
ncbi.nlm.nih.gov
Microvascular
networks (MVNs) formed via endothelial cell self-assembly in 3D
hydrogels have emerged as a widely used platform for modeling
vascularized tissues and studying vascular pathophysiology.
Conventional MVN systems incorporate supporting fibroblasts and may
include biochemical cues such as VEGF, [...]
|
|
|
ncbi.nlm.nih.gov
Microphysiological
systems (MPS) are promising technologies that can enhance the drug
development pipeline and fill gaps in identifying medical
countermeasures for a variety of public health contexts. The
integration of immune cells with MPS is increasingly recognized as a
critical [...]
|
|
|
mdpi.com
Mechano-Organ-on-Chip (Mechano-OoC) platforms are emerging
as powerful microphysiological systems that place mechanical cues at
the center of tumor modeling, providing a scalable and human-relevant
approach to recapitulate the biophysical complexity of the tumor
microenvironment. Mechanical factors such as matrix stiffness,
viscoelasticity, solid stress, interstitial flow, confinement, and
shear
|
|
|
ncbi.nlm.nih.gov
Despite fast-paced
advances in bioengineering human-based models, emulating fine-tuned
and interdependent immunoregulations of the human body remains
challenging. While many bioengineered models contain immune cells,
secondary lymphoid tissues are typically missing. Here, we report a
self-assembled, minimally functional lymphoreticular unit [...]
|
|
|
ncbi.nlm.nih.gov
Engineering
functional arterial tissues in vitro requires dynamic cues that
recapitulate native mechanical environments. A bidirectional
stimulation approach, termed blood and tissue side stretch (BTS), is
presented, applying cyclic circumferential stretch to drive
maturation of arterial microphysiological systems (aMPSs). These
[...]
|
|
|
ncbi.nlm.nih.gov
Organ-on-chip (OoC)
technology offers a more relevant gut model in comparison with static
in vitro gut models from stable cell lines by replicating the 3D
structure, mucus production, fluid dynamics, and mechanical forces,
closely simulating in vivo conditions. Here, we [...]
|
|
|
ncbi.nlm.nih.gov
The blood-brain
barrier (BBB) remains a major obstacle to effective treatment of
central nervous system (CNS) disorders by limiting the entry of most
therapeutics into the brain. The human cerebral microvascular
endothelial cell line (hCMEC/D3) is widely used as an [...]
|
|
|
ncbi.nlm.nih.gov
This study aimed to
reconstruct the hypothalamic-pituitary axis using an organ-on-a-chip
(OOC) model and to evaluate the modulatory role of phoenixin-20 (PNX)
in the regulation of the gonadotrophic axis in sheep. Sixteen female
Polish Merino lambs were used as tissue [...]
|
|
|
ncbi.nlm.nih.gov
There is a growing
demand for automated organ-on-a-chip platforms that are compatible
with off-the-shelf robotic liquid-handling systems and plate readers
to improve reproducibility and scalable analysis. In this work, we
present an end-to-end automated method for fabricating tubular blood
vessel [...]
|
|
|
ncbi.nlm.nih.gov
The drug development
process in cancer faces significant challenges due to high failure
rates in translational studies despite promising in vitro results.
Additionally, conventional animal models exhibit inherent limitations
and ethical concerns, constraining their relevance to cancer studies.
Recognizing the [...]
|
|
|
nature.com
Liver organoids have
emerged as an innovative three-dimensional model system that
effectively recapitulates the structural and functional complexities
of human liver tissue, addressing limitations of traditional
two-dimensional culture systems. This study presents a comprehensive
temporal proteomic characterization of iPSC-derived human liver
organoids (iHLOs) by profiling 5,736 proteins across
|
|
|
ncbi.nlm.nih.gov
In two-dimensional
culture, endothelial cells grow as a monolayer on a flat surface, as
a monolayer monoculture or as co-culture. This type of culture has
the disadvantage that it lacks resemblance to the physiological
conditions of an organism and the [...]
|
|
|
ncbi.nlm.nih.gov
Head and neck
squamous cell carcinoma (HNSCC) presents significant therapeutic
challenges owing to its elevated recurrence rate and resistance to
chemotherapeutic interventions. Tumor organoid models serve as
essential platforms for investigating tumor physiology and
pathological functions in vivo for its [...]
|
|
|
ncbi.nlm.nih.gov
Stem cells are the
basis of organogenesis and regeneration, providing cellular support
during the development, maintenance, and repair of tissues. This
review provides a brief overview of the major stem cell types and
their sources, as well as the key [...]
|
|
|
ncbi.nlm.nih.gov
Endometriosis is a
complex condition affecting 10% of reproductive-age women worldwide,
yet its study has long been hindered by the shortcomings of
traditional research models. This review aims to propose a novel
theoretical framework and strategic roadmap to accelerate precision
[...]
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|
|
ncbi.nlm.nih.gov
The emergence of
organoids has received widespread attention for faithful
recapitulation of physiological and pathological conditions, together
with overcoming the major bottleneck of extrapolating laboratory
findings from model systems to human organs. Organoid technologies
are one of the most revolutionary [...]
|
|
|
link.springer.com
Purpose of Review
Preclinical models are vital to translational breast cancer research.
This review compares cell-line-derived xenografts (CDX) and
patient-derived xenografts (PDX), summarising methodological
advances, strengths and weaknesses, and their applications in
precision oncology. Recent Findings Since 2015, the field has shifted
from CDXs toward PDXs. CDX publications peaked in 2019
|
|
|
ncbi.nlm.nih.gov
The investigation of
breast cancer onset and progression has greatly benefited from the
development of three-dimensional (3D) in vitro models, which enable a
more physiologically relevant representation of the tumor
microenvironment (TME). Over the past decades, several strategies
have been [...]
|
|
|
ncbi.nlm.nih.gov
3D human stem
cell-based brain models are capturing significant interest within the
scientific community and general population. Up to date, a tremendous
portion of study results cannot be translated to clinics due to poor
study models, like over-simplified 2D cell-culture [...]
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link.springer.com
The oviduct is an
important reproductive organ that fosters fertilization and early
embryo development in mammals. With growing utilization of assisted
reproductive technologies (ARTs) in animal agriculture, recent
advancements in cell culture modeling have the potential to optimize
in vitro fertilization methods. Organoids are a three-dimensional
(3D) cell culture model poised to serve a key
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nature.com
During development
of eutherian females, one of the X-chromosomes is silenced. Here,
authors show that alleles on the inactive X-chromosome are
dynamically expressed along neural differentiation enhancing
resilience of female neural tissue. While random X-chromosome
inactivation in female cells of placental mammals silences one allele
of the majority of X-chromosomal genes, a considerable
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nature.com
All cancers arise
from the malignant transformation of normal cells, yet their
cells-of-origin remain challenging to identify due to the inability
to directly observe dynamic changes in human tumors. Retinoblastoma
(Rb), a malignant intraocular cancer, serves as a well-established
model for investigating the molecular and cellular mechanisms
underlying tumorigenesis. While the maturing cone
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ncbi.nlm.nih.gov
Lineage tracing at
single-cell resolution is vital for mapping cell fate decisions, yet
synthetic barcoding faces limitations in precision, diversity, and
toxicity-especially in human pluripotent stem cells (hPSCs). Here, we
repurpose naturally occurring somatic mutations in mitochondrial
transcripts from single-cell [...]
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nature.com
Biallelic
loss-of-function variants of Seizure Threshold 2 (SZT2) cause
neurodevelopmental diseases with developmental delay, epilepsy, and
macrocephaly. SZT2 forms the KICSTOR complex, which represses the
mechanistic target of rapamycin complex 1 (mTORC1) amino
acid-sensitive pathway. SZT2 dysfunction is thought to cause abnormal
activation of the mTOR pathway, underlying the pathogenesis of
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ncbi.nlm.nih.gov
Retinal organoids
are widely used to model human retinal development and disease, but
their utility is limited by the absence of vascular networks and
stable axonal projections, which contribute to retinal ganglion cell
degeneration and loss of function. To address [...]
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ncbi.nlm.nih.gov
3D bioprinting has
revolutionized wound management by addressing the clinical challenges
posed by chronic wounds and acute skin defects. While traditional
skin substitutes serve critical roles in wound healing, current
research focuses on optimizing bioprinting techniques, bioink
formulations, and functional [...]
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link.springer.com
Brain organoids have
revolutionized neuroscience by providing unique in vitro models for
studying human brain development and disease. This study presents a
comprehensive bibliometric analysis of brain organoid research from
2015 to 2025, integrating VOSviewer and CiteSpace, while using
Scimago Graphica to enhance the visualization. Our journal analysis
reveals Stem Cell Reports and Cell Stem
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onlinelibrary.wiley.com
This review explores
the convergence of stem cell biology and bioengineering in gastric
cancer organoid models. We highlight advanced engineering strategies,
including CRISPR genomics, matrix innovations, co‐culture systems,
and microphysiological systems that are used to overcome conventional
limitations. We also examine how integrating high‐throughput data
from these models with artificial
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ncbi.nlm.nih.gov
The human
endometrium is a regenerative tissue essential for fertility, but
pathological conditions like Asherman syndrome, endometrial atrophy,
and thin endometrium can impair its function. Current therapies lack
efficacy, driving demand for innovative regenerative therapies. In
this context, endometrial-derived hydrogels [...]
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ncbi.nlm.nih.gov
Organoid formation
is driven by poorly understood intrinsic cellular properties and
transcriptional programs that govern plasticity and differentiation.
Deciphering these regulatory networks is essential for understanding
normal tissue homeostasis and tumor initiation. Using a 3D
organotypic model, which better recapitulates [...]
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nature.com
CRISPR-based genome
editing therapeutics are entering the clinic, but in vitro and in
vivo tools are needed to assess their safety and efficacy. The
authors review complementary technologies to monitor the biological
effects of genome editing across scales, including the direct
measurement of editing outcomes in DNA, human microphysiological
systems and non-invasive in vivo imaging. CRISPR-based
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onlinelibrary.wiley.com
ABSTRACT
Three‐dimensional, self‐organizing structures derived from stem
cells, known as organoids, represent a groundbreaking advancement in
preclinical drug development. Organoid‐based platforms advance
preclinical testing by providing an accurate representation of human
tissue architecture and genetics, surpassing traditional
two‐dimensional cultures and animal models in testing both
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ncbi.nlm.nih.gov
Venous return
depends on compliant vessels, competent valves, and auxiliary pumps
such as the calf and thoracic muscles. Disruption of these
mechanisms, through immobility, valve dysfunction, or inflammation,
creates disturbed flow and heterogeneous shear that drive venous
dysfunction and disease [...]
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ncbi.nlm.nih.gov
Patient-derived
human organoids have the capacity to self-organize into more complex
structures. However, to what extent gastric organoids can
recapitulate differentiated cell types and mucosal functions remains
unexplored. Here we report on how region-specific gastric organoids
can self-assemble into complex [...]
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mdpi.com
Atherosclerosis
remains a leading cause of cardiovascular mortality despite advances
in pharmacological and interventional therapies. Current treatment
approaches face limitations including systemic side effects,
inadequate local drug delivery, and restenosis following vascular
interventions. Gel-based technologies offer unique advantages through
tunable mechanical properties, controlled
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nature.com
Lung adenocarcinoma
(LUAD) is associated with high morbidity and mortality, making the
discovery of novel and effective drugs treatment crucial.This study
aimed to identify and evaluate antitumor saponin monomers with better
effects using patient-derived organoid (PDO) models from patients
with LUAD. We successfully constructed LUAD PDO models and confirmed
their similarity to original tissues
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onlinelibrary.wiley.com
Biomimetic woven
bone organoids are engineered by embedding multiple MSC spheroids
within a single construct to mimic the fusion of ossification
centers. These organoids maintain stemness while promoting osteogenic
differentiation, exhibit prolonged viability, and enhance bone
regeneration, offering a versatile platform for in vitro modeling and
therapeutic applications.
ABSTRACT
Woven bone is
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ncbi.nlm.nih.gov
Cerebellar organoids
present promising tools for the modelling of human cerebellar
development and diseases. As this young field grows, robust standards
and transparent reporting practices are needed to ensure the
reproducibility and utility of the generated cerebellar organoid
models. Here, [...]
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ncbi.nlm.nih.gov
Human cardiac
organoids have revolutionized the study of cardiac development,
disease modeling, drug discovery, and regenerative therapies. This
review systematically discusses strategies and progress in the
construction of cardiac organoids, categorizing them into three main
types: cardiac spheroids, self-organizing/assembloid organoids,
[...]
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ncbi.nlm.nih.gov
Human pluripotent
stem cell-derived cochlear organoids are promising tools for studying
human cochlear development and disease. However, the maturation of
hair cells in these organoids is slow, requiring 200 days to express
the mature outer hair cell marker Prestin. To [...]
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ncbi.nlm.nih.gov
Persistent Organic
Pollutants (POPs) pose significant global environmental health risks
due to their persistence, bioaccumulation, and long-range transport.
Exposure to POPs has been linked to a range of neurological
disorders, including cognitive impairment, neurodevelopmental
abnormalities, and neurodegenerative diseases. This review
[...]
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ncbi.nlm.nih.gov
Transcription
factors (TFs) mediate gene expression changes during differentiation
and development. However, how TF biophysical properties and abundance
dynamically regulate specific cell state transitions remains poorly
understood. Using automated live-cell single-molecule tracking (SMT)
in intestinal organoid models, we revealed an [...]
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ncbi.nlm.nih.gov
Murine cerebral
organoids provide a rapid and reproducible in vitro system that
recapitulates key aspects of neurogenesis. While human cerebral
organoid protocols are well established, methods for non-human models
remain limited. Here, we present a protocol for generating long-term
cultured [...]
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ncbi.nlm.nih.gov
Regenerative
medicine promises the possibility of custom-made, ready-to-use human
organs without the risk of immune rejection. Human pluripotent stem
cells (hPSCs) are the workhorses of stem cell-based tissue
engineering. With inherent capabilities to adopt nearly any cellular
form, they are [...]
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ncbi.nlm.nih.gov
Understanding the
complexities of the human brain development remains one of the most
formidable challenges in neuroscience, constrained by the limitations
of traditional models and the inaccessibility of brain tissue. The
advent of cerebral organoids has provided a transformative in
[...]
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ncbi.nlm.nih.gov
Biobanks serve as
the cornerstone of translational research. Evolving from traditional
biobanks, living biobanks-particularly organoid living biobanks-have
emerged as a critical and powerful platform, characterized by their
three-dimensional biomimetic architecture, long-term self-renewal
capacity, and retention of key genetic and pathological [...]
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ncbi.nlm.nih.gov
Organoids offer
superior physiological relevance over traditional 2D and animal
models. By recapitulating human tissue architecture, they enable
predictive assessments of drug efficacy, toxicity, and
pharmacokinetics (DMPK). Integrating artificial intelligence
(AI)-driven modeling further expands their preclinical utility,
particularly in oncology. [...]
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ncbi.nlm.nih.gov
Organoids derived
from pluripotent stem cells have emerged as powerful models to study
human development. To investigate signaling pathways regulating human
pancreas differentiation and morphogenesis, we developed a
high-content, image-based screen and quantitative multivariate
analysis pipelines robust to heterogeneity to [...]
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ncbi.nlm.nih.gov
Previous animal
models of sporadic Alzheimer's disease (sAD), based on the β-amyloid
(Aβ) cascade hypothesis and induced by Aβ1-42 oligomers (AβO),
only recapitulated early AD pathological features. sAD cerebral
organoids (COs) model employed Aβ coculture approach and found no
typical [...]
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ncbi.nlm.nih.gov
Tumor stem cells
(TSCs) play a pivotal role in the development of tumor organoids.
Consequently, the development of effective methods for the isolation
and differential induction of TSCs is essential for the establishment
of tumor organoids. In this study, we [...]
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ncbi.nlm.nih.gov
Lung cancer remains
a significant global health challenge, with metastatic progression
being the leading driver of mortality. Organoid technology provides a
tractable, physiologically relevant platform to model key aspects of
lung cancer metastasis in vitro. The present review summarized
methodologies [...]
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ncbi.nlm.nih.gov
Reconstructing
networks of neurons in vitro is essential for advancing our
understanding of functional mechanisms and disease pathogenesis.
However, neuronal culture methods including organoids are limited in
network structure complexity required for their functionality and
dynamics. In this study, we [...]
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ncbi.nlm.nih.gov
Lung cancer, the
leading cause of cancer-related mortality, presents major challenges
for both standard therapies and chimeric antigen receptor (CAR) T
cell therapy due to tumour heterogeneity and resistance. Preclinical
models that capture patient-specific factors are essential for
personalizing treatment [...]
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link.springer.com
The integration of
next-generation sequencing (NGS) has elevated organoid research
significantly and was instrumental in making patient-derived
organoids (PDOs) a promising tool for biomarker discovery, drug
response analysis in vivo, and personalized treatment strategies. On
one hand, high-throughput capabilities have made NGS invaluable in
comparing organoids with parental tumors they were
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ncbi.nlm.nih.gov
Cancer and tissue
regeneration pose great challenges to global health, as cancer
treatment is impeded by tumor heterogeneity and therapy resistance,
while regenerative medicine is constrained by donor shortages and
difficulties in replicating native tissue structures. Organoids, as
advanced three-dimensional [...]
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ncbi.nlm.nih.gov
Large-cohort
genome-wide association studies (GWAS) for alcohol use disorder (AUD)
drug treatment outcomes and AUD risk have repeatedly identified
genetic loci which are splicing quantitative trait loci for the
fibronectin III domain containing 4 (FNDC4) gene in the brain.
However, [...]
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ncbi.nlm.nih.gov
Human liver ductal
epithelium is morphologically, functionally, and transcriptionally
heterogeneous. Understanding the impact of this heterogeneity has
been challenging due to the absence of systems that recapitulate this
heterogeneity in vitro. Here, we found that human liver cholangiocyte
organoids do [...]
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ncbi.nlm.nih.gov
A major obstacle to
identifying effective therapies for the aggressive brain tumor
glioblastoma is the lack of human-specific, immunocompetent models
that reflect the human tumor microenvironment. To address this, we
developed the immune-human organoid tumor transplantation (iHOTT)
model, an autologous [...]
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ncbi.nlm.nih.gov
We measured genomic
responses to active vitamin D, 1α,25-dihydroxyvitamin D (1,25D), in
colonic organoids from individuals of African and European ancestry.
Given protective effects of 1,25D for gastrointestinal conditions
such as colorectal cancer, organoid cultures enabled evaluation of
condition-specific responses [...]
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link.springer.com
Urogenital cancers,
including prostate, kidney, and bladder cancer, remain a significant
clinical challenge due to their high incidence, molecular
heterogeneity, and frequent resistance to standard therapies. Despite
progress in genomic profiling and precision oncology, the translation
of molecular data into effective therapeutic decisions remains
limited by the lack of functional models capable
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link.springer.com
Organoid technology
integrates stem-cell biology, biomaterials, and engineered culture
platforms to recreate human tissue architecture with greater
physiological relevance than 2D cultures or animal models. Advances
in stem-cell differentiation and matrix design have facilitated the
formation of complex, organ-specific 3D structures. This review
presents the latest strategies for generating PSC
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nature.com
3D cell culture
systems such as organoids reflect the complexity of native organs
more closely than traditional 2D culture systems. However, efforts to
translate organoid research into clinical and industrial applications
demand the development of compatible analytical platforms to
accurately characterize their multifaceted physiological traits. This
Review explores three vital aspects of
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ncbi.nlm.nih.gov
Engineering
functional arterial tissues in vitro requires dynamic cues that
recapitulate native mechanical environments. A bidirectional
stimulation approach, termed blood and tissue side stretch (BTS), is
presented, applying cyclic circumferential stretch to drive
maturation of arterial microphysiological systems (aMPSs). These
[...]
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ncbi.nlm.nih.gov
Pancreatic ductal
adenocarcinoma (PDAC) is one of the most lethal malignancies with a
5-year survival rate of < 13%. Standard treatments such as FOLFIRINOX
or gemcitabine/nab-paclitaxel yield modest response rates,
underscoring the urgent need for precision oncology approaches.
Patient-derived organoids [...]
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ncbi.nlm.nih.gov
Rare vascular
diseases are a diverse group of life-threatening conditions defined
by their low prevalence but profound impact on patient morbidity and
quality of life. Diagnosing these disorders remains a significant
clinical challenge due to their genetic heterogeneity, overlapping
phenotypes, [...]
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link.springer.com
Pediatric cancers
present unique biological challenges and remain a leading cause of
disease-related death in children. While treatment advances have
improved outcomes, progress is limited for many high-risk and
relapsed tumors. Organoid technology—three-dimensional cultures
derived from patient or stem cells—offers a powerful new platform
to model pediatric tumors more accurately. This
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ncbi.nlm.nih.gov
Organoid technology
has become among the most popular technologies in recent years, due
to their three-dimensional and physiologically enriching models that
closely mimic the structure and function of human organs. Herein,
this review details the in-depth methodology, updated to date,
[...]
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link.springer.com
Organoids lie in the
middle ground between the full complexity of in vivo organs and
traditional two-dimensional (2D) cell cultures. Due to this, organoid
models allow researchers to alter the genetic backgrounds of cells
more easily, less expensively, and more quickly than generating new
mouse models. The advantages of an organoid model vs. an in vivo
model may be more obvious than that of a
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ncbi.nlm.nih.gov
Advances in stem
cell biology and tissue engineering have enabled the generation of
brain organoids from human induced pluripotent stem cells (hiPSCs),
providing powerful platforms to model human brain development and
neurodegenerative diseases. However, the lack of vascularization in
conventional [...]
|
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ncbi.nlm.nih.gov
Organoid research
offers valuable insights into human biology and disease, but
reproducibility and scalability remain significant challenges,
particularly for epithelial organoids. Here, we present an integrated
microfluidic platform that addresses these limitations by enabling
high-throughput generation of uniform hydrogel microparticles
[...]
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ncbi.nlm.nih.gov
While tumor
organoids hold promise for personalized medicine, clinical validation
of epithelial ovarian cancer (EOC) organoids as predictors of
therapeutic efficacy-particularly for PARP inhibitors (PARPi)-remains
unestablished.
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link.springer.com
Purpose Current lung
cancer organoid models often fail to replicate the complex tumor
immune microenvironment, reducing their predictive value for
immunotherapy and radiotherapy. Therefore, it is crucial to establish
an optimized lung cancer organoid model which could recapitulate the
tumor immune microenvironment, enabling more accurate evaluation of
therapeutic responses. Methods We developed
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nature.com
Background A
minority of Colorectal Cancer (CRC) patients with peritoneal
metastases is eligible for cytoreductive surgery (CRS) followed by
hyperthermic intraperitoneal chemotherapy (HIPEC). However,
recurrence rates are high (~80%). We tested whether inhibitors of the
DNA damage response (DDR) could prevent recurrence in an in vitro
HIPEC model. Methods Peritoneal metastasis-derived organoids
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link.springer.com
Cardiovascular
disease (CVD) is the leading cause of death globally, highlighting
the urgent need for more accurate and human-relevant research models.
Traditional in vitro and animal models have proven to be insufficient
in replicating the structural and functional complexity of the human
heart. Recent advances in stem cell biology and tissue engineering
have facilitated the development of human
|
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link.springer.com
Over the last
10 years, patient-derived tumor organoids (PDTOs) have significantly
expanded the range of preclinical models and gradually transformed
the landscape of three-dimensional cell culture in cancer research.
PDTOs can be efficiently generated from patient tumor samples and
accurately reproduce the histological and molecular features of the
original tumor. As a result, PDTOs represent
|
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nature.com
Diffuse midline
glioma (DMG) is a highly aggressive and untreatable pediatric cancer
primarily arising in the pontine brainstem region, necessitating the
development of representative models for treatment advance. Here we
developed an FGF4-driven human brainstem organoid model, which we
used to genetically engineer H3.3K27M-altered DMG. We demonstrated
that brainstem pontine glial specification
|
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ncbi.nlm.nih.gov
Previous studies on
hematopoiesis were mainly conducted in model animals. However, direct
investigation of human hematopoiesis remains challenging due to
limited access to human samples and ethical concerns. Traditional
two-dimensional culture systems have provided valuable opportunities
to study human hematopoiesis, [...]
|
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ncbi.nlm.nih.gov
Human pluripotent
stem cell (hPSC)-derived GABAergic neurons offer potential for
treating neurological disorders by restoring disrupted inhibitory
circuits, yet current differentiation methods show limited
efficiency, purity, and subtype specificity. We present an approach
for generating lateral/caudal ganglionic eminence (LGE/CGE) organoids
[...]
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onlinelibrary.wiley.com
Micro‐ and
nanoengineering have revolutionized cardiac tissue engineering by
overcoming the structural and functional limitations of conventional
methods, enabling precise remodeling of the heart's native
microenvironment. Use of Micro/nanoengineering strategies including
microwells, microfluidics, conductive nanomaterials, and embedded
biosensors enhance both spheroid and organoid platforms by
|
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ncbi.nlm.nih.gov
Introduction Otic
organoids differentiated from human pluripotent stem cells are
three-dimensional in vitro cultures that broadly mimic the complexity
and functionality of the human inner ear. They provide a valuable
model for developmental and disease-related studies. However, current
protocols differ [...]
|
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ncbi.nlm.nih.gov
Human
cytomegalovirus (HCMV) is the leading viral cause of congenital
defects. The triggers of viral neuropathogenesis during congenital
infection (cCMV) are still unclear, and treatment options are
limited. We used both a two-dimensional model of dynamic neurogenesis
and cerebral organoids [...]
|
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ncbi.nlm.nih.gov
PARP inhibitors
(PARPis) are new targeted agents that exploit homologous
recombination DNA repair deficiencies (HRDs), which are present in
50% of high-grade serous carcinoma (HGSC) cases. Currently, olaparib
is approved as maintenance therapy for BRCA1/2 -mutated HGSC, and
niraparib is [...]
|
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ncbi.nlm.nih.gov
Organoids are
three-dimensional cellular structures formed through the
self-organization of stem cells that mimic the structure and function
of in vivo organs, offering broad applications in biomedical
research. Mesenchymal stem cells (MSCs) are multipotent cells
characterized by their self-renewal capacity [...]
|
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ncbi.nlm.nih.gov
Liu et al. 1
generate human sympathetic ganglion organoids and assemble them with
heart-forming organoids to build functionally connected
sympathetic-cardiac organoids in vitro. Their platform captures
autonomic development, neuromodulation of heart function, and
metabolic injury, opening new opportunities to study [...]
|
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ncbi.nlm.nih.gov
Human pineal
development and neuroendocrine regulation remain incompletely
understood at the molecular and functional levels. Kiral et al. 1
establish human pineal gland organoids (hPGOs) that recapitulate
pinealocyte maturation, noradrenergic responsiveness, and melatonin
synthesis, providing a platform for investigating circadian
[...]
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ncbi.nlm.nih.gov
Brain tumors are the
most common solid cancer in children, with radiotherapy being a
primary treatment. Proton therapy, with its precise dose
distribution, is increasingly being used in these patients to
minimize damage to the developing brain. However, the biological
[...]
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ncbi.nlm.nih.gov
Invariant NKT (iNKT)
cells have a T-cell receptor that is common to all individuals and
are activated by recognizing glycolipids on MHC class I-like CD1d
molecules. Activated iNKT cells are known to exert anti-tumor effects
through the activation of other [...]
|
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nature.com
High pathogenicity
avian influenza viruses pose a growing threat to poultry, livestock,
wildlife, and humans as they undergo accelerated expansion of host
and geographical ranges. Since 2020, these viruses have driven a
panzootic characterized by extensive viral diversification and
spillover into species previously considered to be resistant. There
is currently a lack of physiologically relevant
|
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onlinelibrary.wiley.com
Upon targeted
binding to CD47‐overexpressing colorectal cancer (CRC) cells, the
nanoparticles disrupt signal regulatory protein α‐CD47 signaling
and allow for the restoration of innate immune response by
tumor‐associated macrophages (TAMs) against CRC. The nanoparticles
are also endocytosed by the cancer cells and steadily release
cisplatin, which generates reactive oxygen species and
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onlinelibrary.wiley.com
Schematic
representation of M@E@CF nanosensors for detecting vesicular storage
and release in cholinergic neurons and brain organoids. (A)
Nano‐tip microelectrodes modification via molds fabricated through
3D printing. (B) the reaction mechanism for acetylcholine detection
at the electrode interface. (C) Quantification of acetylcholine
storage (a) in vesicles of cholinergic neurons and
|
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|
onlinelibrary.wiley.com
Schematic
representation of M@E@CF nanosensors for detecting vesicular storage
and release in cholinergic neurons and brain organoids. (A)
Nano‐tip microelectrodes modification via molds fabricated through
3D printing. (B) the reaction mechanism for acetylcholine detection
at the electrode interface. (C) Quantification of acetylcholine
storage (a) in vesicles of cholinergic neurons and
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link.springer.com
Bone tumor organoids
(BTOs) are three-dimensional culture systems developed from
patient-derived tissues, which accurately replicate the growth
dynamics and cellular interactions characteristic of bone tumors in
vitro. Organoids offer improved physiological relevance compared to
traditional models, thereby enhancing the precision of therapeutic
strategies and addressing existing treatment
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onlinelibrary.wiley.com
A unidirectional
cerebral organoid–organoid neural circuit is established using a
microfluidic platform, enabling controlled directional propagation of
electrical signals, neuroinflammatory cues, and neurodegenerative
disease–related proteins between spatially separated organoids.
This system provides a versatile in vitro model to study
unidirectional neural connectivity and pathological
|
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ncbi.nlm.nih.gov
CFTR modulating
therapies have advanced significantly but remain ineffective for
people with CF (pwCF) with mutations preventing CFTR protein
production. PwCF carrying non-canonical splicing mutations show
modest benefits. SPL84, an antisense oligonucleotide (ASO) developed
by Splisense, targets the non-canonical CFTR [...]
|
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ncbi.nlm.nih.gov
Protein phase
separation has emerged as a crucial mechanism for spatiotemporal
regulation of intracellular processes, yet its potential to integrate
and compute diverse extracellular signals is not fully understood.
Here, we show a mechano-biochemical circuit that harnesses phase
separation to [...]
|
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ncbi.nlm.nih.gov
Diabetic kidney
disease (DKD) is one of the most pervasive complications of diabetes
worldwide. However, the pathogenesis of DKD remains poorly
understood, due to limitations of the models. The hPSC-derived kidney
organoids may offer a new possibility to solve the [...]
|
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|
ncbi.nlm.nih.gov
The goal of
engineering increasingly complex human organoid models is to more
accurately model human organogenesis and disease. Recently, human
antral gastric organoids (hAGOs) were engineered to contain
splanchnic mesenchyme (SM) and enteric neural crest cells (NCCs),
resulting in functional [...]
|
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|
ncbi.nlm.nih.gov
Developmental tissue
engineering (TE) strategies recapitulating endochondral ossification
(ECO)-the major ossification pathway in bone development and repair-
are a promising avenue for the treatment of critical size and
congenital bone defects. In this work, we develop a customizable
approach using [...]
|
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nature.com
The intestinal
epithelium plays a critical role in health and disease, yet the
impact of microenvironmental cues in diseased contexts, such as
inflammatory bowel disease (IBD), remains poorly defined. To address
this gap, we first benchmarked human colonic organoid injury models
against IBD tissue and established a disease-relevant model of
inflammation using inflammatory cytokines. Using this
|
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ncbi.nlm.nih.gov
Three-dimensional
(3D) culture systems provide physiologically relevant models that
better replicate in vivo cell architecture and function compared to
the conventional two-dimensional cultures. In reproductive biology,
3D models of endometrial cells have emerged as powerful tools to
investigate uterine physiology, [...]
|
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ncbi.nlm.nih.gov
Moyamoya disease
(MMD) is a cerebrovascular disorder characterized by progressive
intracranial arterial stenosis, which can lead to ischemic or
hemorrhagic stroke. However, its pathogenesis remains poorly
understood, limiting advances in the diagnosis and treatment of MMD.
We perform data-independent acquisition [...]
|
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ncbi.nlm.nih.gov
Neural organoids
(NOs) have emerged as important tissue engineering models for
microphysiological systems, brain sciences, and biocomputing.
Establishing reliable relationships between stimulation and recording
traces of electrical activity is essential for monitoring the
functionality of NOs, especially in paradigms such [...]
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